Veysel Atilla AYYILDIZ, Rahime ASLANKOÇ, Saygın MUSTAFA, Demet GÜNDÜZ

Bilgisayarlı Tomografi İle Yapılan Perkütan Torasik Kitle Biyopsilerinde Lenfositlerdeki DNA Hasarının Değerlendirilmesi

Amaç: Son yüzyılda radyasyonun tıbbi ve endüstriyel kullanımlarındaki artış, insanların daha yüksek dozlarda radyasyona maruz kalmasına neden olmuştur. Yüksek doz radyasyonun insan sağlığına zararları bilinmesine rağmen düşük doz radyasyonun sağlık üzerindeki etkileri henüz tam olarak aydınlatılamamıştır. Bu çalışmada, bilgisayarlı tomografi (BT) ile perkütan torasik kitle biyopsisi planlanan akciğer, plevra/torasik duvar kitleleri olan hastalarda lenfositlerdeki DNA hasarını araştırmayı amaçladık. Materyal Metod: Bir kamu kurumu hastanesinin Radyoloji Kliniğine akciğer, plevra/torasik duvar kitleleri ile başvuran ve BT eşliğinde perkütan biyopsi planlanan 16 hasta çalışmaya dahil edildi. Tüm biyopsiler 128 kesitli CT cihazı (Definition AS, Siemens Medical Solutions, Forchheim, Germany) ile yapıldı. Hastalardan biyopsi öncesi ve sonrası alınan venöz kan örneklerinde comet assay ile lenfosit analizi yapıldı. DNA hasarı, görüntüleme analiz yöntemi ile kantitatif olarak değerlendirildi. Bulgular: Çalışma grubunun BT analiz verileri değerlendirildiğinde; ortalama tarama mesafesi 19,92±3,60 sn, ortalama toplam miliamper-saniye 807,43±304,51 ve ortalama doz-uzunluk çarpımı 765,44±278,36 mGy.cm olarak bulundu. İşlem öncesi göç yapan hücrelerde ortalama comet skoru 200,50±40,54 ve işlem sonrası göç yapan hücrelerde 237,37±27,85 olarak tespit edildi. İşlem sonrası comet skorları, işlem öncesi comet skorlarına göre anlamlı olarak arttı (p=0,038). Sonuç: BT eşliğinde perkütan biyopsi yapılan hastalarda işlem sonrası DNA hasarı saptandı.

Anahtar Kelimeler:

Comet assay, DNA hasarı, İyonlaştırıcı radyasyon, Bilgisayarlı tomografi

Evaluation of DNA Damage in Lymphocytes in Percutaneous Thoracic Mass Biopsies Performed with Computed Tomography

Purpose: The increase in the medical and industrial uses of radiation in the last century has caused people to be exposed to higher doses of radiation. Although the harms of high-dose radiation on human health are known, the effects of low-dose radiation on health have not yet been fully elucidated. In this study, we aimed to investigate DNA damage in lymphocytes in patients with lung, pleura/thoracic wall masses planned for percutaneous thoracic mass biopsy with computed tomography (CT). Methods: Sixteen patients referred to the Radiology Clinic of a public institution hospital with lung, pleura/thoracic wall masses and scheduled to undergo a CT-guided percutaneous biopsy were included in the study. All the biopsies were performed with a 128-slice CT device (Definition AS, Siemens Medical Solutions, Forchheim, Germany). Lymphocytes were analyzed using the comet assay in the venous blood samples taken from the patients before and after the biopsy procedure. DNA damage was quantitatively evaluated with the imaging analysis method. Results: In the CT analysis data of the study group, the mean scan distance was found to be 19.92±3.60 sec, the mean total milliampere-seconds was 807.43±304.51, and the mean dose-length product was 765.44±278.36 mGy.cm. The mean comet score was 200.50±40.54 for the cells that migrated before the procedure and 237.37±27.85 for those migrating after the procedure. The post-procedure comet scores significantly increased compared to the pre-procedure comet scores (p=0.038). Conclusion: Post-procedure DNA damage was detected in patients who underwent CT-guided percutaneous biopsy.

Keywords:

Comet assay, DNA damage, Ionizing radiation, Computed tomography,

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