Özcan EREL, Mehmet ŞAHİN, Ayşe BALKARLI, Gözde YILDIRIM ÇETİN, Salim NEŞELİOĞLU, Atalay DOĞRU

Ailesel Akdeniz Ateşi olan hastalarda dinamik tiyol / disülfid homeostazının değerlendirilmesi

Amaç: Ailesel Akdeniz Ateşi (AAA), ateş ile birlikte tekrarlayan serozal inflamasyon ataklarını içeren, kalıtsal bir otoinflamatuar hastalıktır. Oksidatif stresin belirteci Tiyol / disülfid homeostazı, birçok inflamatuar hastalıkta inflamatuar sitokinlerde artış ile ilişkilidir. Çalışmamızın amacı atak dışı dönemde AAA olan hastalarda dinamik tiyol / disülfid homeostazisini değerlendirmektir. Materyal-Method: Çalışmamıza atak dışı dönemde 60 AAA' li, 60 inaktif dönemde Ankilozan Spondilit (AS)’ li hasta ve 60 yaş ve cinsiyet olarak benzer kontrol grubu dahil edildi. Erel ve Neselioğlu yöntemi kullanılarak nativ tiyol, total tiyol ve disülfid düzeyleri ölçüldü. Bulgular: AAA grubunda total tiyol ve disülfid düzeyleri sağlıklı kontrollere göre anlamlı derecede düşük bulundu. AAA grubunda nativ tiyol düzeylerinin AS grubuna göre anlamlı olarak yüksek olduğu saptandı. Bununla birlikte, AAA ve AS grubu arasında total tiyol düzeylerinde anlamlı bir farklılık izlenmedi. Plazma nativ tiyol, total tiyol ve disülfid seviyeleri kullanılarak elde edilen oranlar, AAA, AS ve kontrol grupları arasında önemli ölçüde farklıydı. Sonuç: AAA hastalarında AS ve control grubuna göre düşük tiyol düzeyi saptanması, farklı patofizyolojiye sahip hastalıklarda tiyol / disülfid dengesinin farklı şekilde etkilendiğini düşündürmektedir. Hastalık klinik olarak inaktif bir dönemde olmasına rağmen, farklı romatizmal hastalıklarda inflamasyon farklı düzeylerde devam edebilir.

Anahtar Kelimeler:

Ankilozan Spondilit, Ailesel Akdeniz Ateşi, Tiyol / Disülfid

Evaluation of dynamic thiol/disulfide homeostasis in patients with Familial Mediterranean Fever

Introduction: Familial Mediterranean fever (FMF) is a hereditary autoinflammatory disease that includes recurrent episodes of serosal inflammation, with accompanying fever. Thiol/disulfide homeostasis, marker of oxidative stress, is associated with an increase in inflammatory cytokines in many inflammatory diseases. The aim was to evaluate the dynamic thiol/disulfide homeostasis in patients with FMF during attack free period.Material and method: In our study, 60 patients with FMF during attack free period, 60 patients with Ankylosing Spondylitis (AS) during inactive period and 60 age-and sex matched controls were included. Native thiol, total thiol and disulfide levels were measured by using the Erel and Neselioglu method. Result: Total thiol and disulfide levels were found to be significantly lower in FMF group compared to healthy controls. Native thiol levels were detected to be significantly higher in FMF group compared to AS group. However, no significant difference was observed in total thiol levels between FMF and AS group. Ratios obtained using plasma native thiol, total thiol, and disulfide levels differed significantly between the FMF, AS and the control groups. Conclusion: The lower thiol level of FMF group compared to AS and controls suggests that the thiol / disulfide balance is affected differently in diseases with different pathophysiology. Although the disease is in clinically inactive period, inflammation may continue at different levels in different rheumatic diseases.

Keywords:

Ankylosing Spondylitis, Familial Mediterranean fever, Thiol/disulfide,

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