Halime Keriman ÇETİNKAYA, Damla GÜVERCİN, Yaşar KARAKURT

Nar (Punica granatum L.) Çeşitlerinin SSR Markörleri ile Moleküler Karakterizasyonu

Nar, Lythraceae familyasına ait tropik ve subtropik iklim kuşağında yetiştirilen bir meyve olarak bilinmektedir. Çalışma kapsamında Akdeniz Bölgesi Antalya ilinde yer alan Batı Akdeniz Tarımsal Araştırma Enstitüsü’nden 10 çeşit nar ve küçük özel mülk bahçesinden 1 çeşit nar alınarak moleküler analizler için kullanılmıştır. Bu amaçla genotiplere ait numuneler uygun koşullarda alındıktan sonra moleküler analizleri gerçekleştirilmiştir. SSR markörleri ile yapılan analizler sonucunda UPGMA kümeleme metoduna göre nar çeşitleri arasında iki ana grup ortaya çıkmış ve %65 oranında benzerlik gösterdiği belirlenmiştir. İlk ana grup kendi içinde 4 alt gruptan meydana gelmiştir. İlk alt grupta Hicaz, ikinci alt grupta Aşınar, Batem Onur, Ernar, Batem Hicaz, üçüncü alt grupta Beynarı ve dördüncü alt grupta Batem Esin, Batem Yılmaz ve Ekşilik yer almıştır. İkinci ana grup 2 alt gruba ayrılmış olup Katırbaşı ile Fellahyemez farklı gruplarda yer almaktadır. Batem Onur ve Ernar çeşitlerini birbirlerinden ayırt edecek polimorfizmler üretilemediğinden bu iki çeşit bir arada gruplanmıştır. Hicaz, Beynarı, Ekşilik, Katırbaşı ve Fellahyemez çeşitleri tek başına bir alt grup oluşturmuştur. Çalışmada Aşınar ve Batem Onur-Ernar, Batem Esin ve Batem Yılmaz arasında yakın genetik benzerlik olduğu gözlemlenmiştir. Nar türüne ait SSR bulgularının, gelecekteki ıslah ve koruma çalışmalarında kullanılabilecek en uygun genotiplerin belirlenmesine yardımcı olacağı beklenmektedir.

Anahtar Kelimeler:

Moleküler karakterizasyon, Markör, SSR, Nar

Molecular Characterization of Pomegranate (Punica granatum L.) Genotypes with SSR Markers

Pomegranate is known to be a fruit grown in tropical and subtropical climate zone belonging to Lythraceae family. Within the scope of the study, 10 kind of pomegranates and a pomegranate from a small private garden have been used for molecular analyzes from the Western Mediterranean Agricultural Research Institute in the Antalya Region. To accomplish this objective, samples of genotypes were taken under appropriate conditions and molecular analyzes were performed. As a result of analysis with SSR markers, pomegranate assortment has emerged as two main groups and 65% of the similarity has been detected between the groups as per the UPGMA method. The first main group consisted of 4 sub-groups. In the first sub-group Hicaz, in the second subgroup Asi, Batem Onur, Ernar, Batem Hicaz, the third sub-group Beynarı and the fourth sub-group Batem Esin, Batem Yılmaz and Ekşilik took place. The second main group is divided into 2 sub-groups. In the first sub-group Katırbaşı and in the second sub-group Fellahyemez took place. Polymorphisms that distinguish Batem Onur and Ernar from each other could not be produced hence these two assortments were grouped together. Hicaz, Beynar, Ekşilik, Katırbaşı and Fellahyemez types formed a subgroup alone. In the study, a close correlation between Aşınar and Batem Onur-Ernar, Batem Esin and Batem Yılmaz has been observed. SSR findings of pomegranate species, creating a step in the selection of the next breeding parents in determining the span of pomegranate genotypes for comparison of the genetic collection can be used in the characterization.

Keywords:

Molecular characterization, Marker, SSRs, Pomegranate,

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