Hale SEÇİLMİŞ CANBAY, Mahmut DOĞANTÜRK, Yusuf YILMAZ

Türk Siyah Çayı, Hazır Kahve ve Türk Kahvesi Örneklerinin Akrilamid İçeriği

Bu çalışmada, Türk ve hazır kahve ile siyah çay örneklerinin akrilamid içerikleri, herhangi bir türevlendirme basamağı olmadan gaz kromatografisi kütle spektrometrisi ile belirlenmiştir. Numune hazırlama yöntemi iki aşamadan oluşmaktadır. İlk basamak, izolasyon aşamasıdır. Bu adımda, etanol ile Carrez I ve II çözeltileri örnek karışımlarına eklenmiştir. Katı faz ekstraksiyonu ikinci adımı oluşturmaktadır. Çalışmada, C18 kartuşu temizleme için kullanılmıştır. Tespit ve tayin limiti sırasıyla 1,11 ve 3,66 ng/mL’dir. Sekiz hazır kahve örneğinde 4,48 ile 15,71 µg/kg arasında değişen akrilamid miktarı belirlenmiştir. Yedi Türk kahvesi numunesi ise, 3,89 ile 88,44 µg/kg arasında değişen yüksek bir akrilamid içeriğine sahiptir. On yedi siyah çay numunesinden sadece beş tanesinde, 7,02 ile 19,85 µg/kg arasında değişen miktarda akrilamid tespit edilmiştir.

Anahtar Kelimeler:

Türk kahvesi, Hazır kahve, Siyah çay, Akrilamid, SPE, GC-MS

Acrylamide Content of Turkish Black Tea, Instant and Turkish Coffee Samples

Acrylamide contents of Turkish and instant coffee and black tea samples were determined by the gas chromatography mass spectrometry without any derivatization step in this present study. The sample preparation method included two primary clean up steps. In the first step, ethanol and solutions of Carrez I and II were added into sample mixtures. The second step with solid-phase extraction C18 cartridge was carried out for clean-up. The limit of detection and limit of quantification were 1.11 and 3.66 ng/mL, respectively. The quantification level of acrylamide was determined in eight instant coffee samples in the range between 4.48 and 15.71 µg/kg while seven Turkish coffee samples had a high content of acrylamide ranging from 3.89 to 88.44 µg/kg. Acrylamide was detected in only five samples from seventeen black tea samples at a quantification level between 7.02 and 19.85 µg/kg.

Keywords:

Turkish coffee, Instant coffee, Black tea, Acrylamide, SPE, GC-MS,

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