Lurus kraepelini (Scorpiones, Luridae) Zehir Bezi Glikoproteinlerinin Histokimyasal Yapısı

Bu çalışmada Lurus kraepelini (von Ubisch, 1922) türü akreplerin zehir bezinin histokimyasal yapısının belirlenmesi amaçlandı. Materyal olarak Isparta’nın Aksu ilçesinden toplanan 50 adet Lurus kraepelini’ye ait telsonlar kullanıldı. Zehir kesesinin histolojik yapısı ve glikoproteinlerin histokimyasal yapısı uygun boyama teknikleri kullanılarak belirlendi. Zehir bezinin genel histolojik yapısında zehir kesesinin iç kısmının bağ dokusundan oluştuğu ve bu bağ dokusu içerisinde zehir salgılayan hücreler, destek hücreleri ve mukus hücrelerinin bulunduğu belirlendi. Histokimyasal boyama yöntemleriyle zehir bezinin sülfatlı ve karboksilli asidik, siyalik asitli, nötr ve asidik mukosubstansa sahip olduğu tespit edilerek zehir bezinin her bölgesindeki glikoproteinlerin farklı karakterlere sahip olduğu anlaşıldı. Sonuç olarak zehir bezinde kitin tabakasının nötr, sülfatlı ve asidik; kas tabakasının ve zehir salgı hücrelerinin nötr; epitel salgı hücrelerinin ise asidik mukosubstansı yoğun olarak içerdiği belirlendi. Buna karşın güçlü sülfatlı, zayıf sülfatlı ve sülfat esterli glikoproteinleri içermediği dikkati çekti.

Anahtar Kelimeler:

akrep, lurus kraepelini, zehir bezi, glikoprotein, histokimya, Scorpion, Lurus kraepelini, Venom gland, Glycoprotein, Histochemistry

Histochemical Structure of Glycoproteins of Lurus kraepelini (Scorpiones, Luridae) Venom Gland

In this study, it was aimed to determine the histochemical structure of the venom gland of Lurus kraepelini (von Ubisch, 1922) scorpions. Telsons belonging to 50 Lurus kraepelini collected from Aksu district of Isparta were used as material. The histological structure of the venom sac and the histochemical structure of the glycoproteins were determined using appropriate staining techniques. In the general histological structure of the venom gland, it was determined that the inner part of the venom sac consisted of connective tissue, and that venom secreting cells, support cells and mucus cells were found in this connective tissue. By histochemical staining methods, it was determined that the venom gland had sulphate and carboxylic acidic, sialic acid, neutral and acidic mucosubstances, and it was understood that the glycoproteins in each region of the venom gland had different characters. As a result, the chitin layer in the venom gland was neutral, sulfated and acidic; neutral of the muscle layer and venom secretion cells; It was determined that epithelial secretory cells contained acidic mucosubstance intensely. On the other hand, it was noted that it did not contain glycoproteins with strong sulfates, weak sulfates and sulfate esters.

Keywords:

Scorpion, Lurus kraepelini, Venom gland, Glycoprotein, Histochemistry,

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