Koyun (Ovis aries) Abomazum (Kardiya, Fundus ve Pilorus) Mukozasının Lektin Histokimyasal Yapısı

Bu çalışmada, koyun (Ovis aries) abomazum (kardiya, fundus ve pilorus) mukozasında bulunan glikokonjugatların, DBA (Dolichos biflous), PSA/PEA (Pisum sativum) ve AIA (Artocarpus integrifolia) lektinleri kullanılarak belirlenmesi amaçlandı. Uygulanan lektin histokimyasal yöntem sonucunda kardiya bölgesinde yüzey epitel hücrelerinin yüzeyinde, apikal sitoplazmalarında, foveola gastrika epitel hücreleri ve bez epitel hücrelerinde DBA, PSA/PEA ve AIA reaksiyonu saptandı. Bez epitel hücrelerinin çekirdeklerinin üstündeki alanda DBA' ya karşı güçlü reaksiyon gözlendi. Fundus bölgesinde yüzey epitel hücreleri ve kollum hücrelerinin apikal sitoplazmalarında DBA, PSA/PEA ve AIA reaksiyonuna rastlandı. Foveola gastrika epitel hücrelerinin çekirdeklerinin üstündeki alanda çok güçlü AIA reaksiyonu saptandı. Fundus bezlerinde prensipal hücrelerin yüzeyinde DBA ve AIA uygulamasında çok güçlü reaksiyon gözlendi. Bezlerin hem yüzeye yakın kısımlarında hem de dip kısımlarda bulunan pariyetal hücrelerde uygulanan üç lektine karşı da reaksiyon belirlendi. Pilorus bölgesinde yüzey epitel hücrelerinin yüzeyinde ve apikal sitoplazmalarında PSA/PEA lektin uygulamasında reaksiyona rastlanmadı. Bez epitel hücrelerinin çekirdeklerinin üstündeki dar alanda DBA ve AIA'ya karşı çok güçlü reaksiyon gözlendi.

Lectin Histochemical Structure of Abomasum (Cardia, Fundus and Pylorus) Mucosa in Sheep (Ovis aries)

In this study, it was aimed the determination of glycoconjugates in mucosa of sheep's (Ovis aries) abomasum (cardia, fundus ve pylorus) using DBA (Dolichos biflorus), PSA/PEA (pisum sativum) and AIA (Artocarpus intergrifolia) lectins. Results of lectin histochemistry revealed that a reaction to DBA, PSA/PEA and AIA lectins occurred in surface epithelial cells and their apical cytoplasm, gastric foveolar epithelial cells and glandular epithelial cells in the cardia region. A strong reaction to DBA was observed in an area above the nucleus of glandular epithelial cells. Similarly, a reaction to DBA, PSA/PEA and AIA lectins was seen in surface epithelial cells and the apical cytoplasm of neck ce[37] lls in the fundus region. A very strong reaction to AIA was determined in an area above the nucleus of glandular epithelial cells. A very strong reaction to DBA and AIA was detected on surface of glandular principal/chief cells. It was established that the parietal cells found in near-surface and bottom parts of the glands reacted with three lectins employed. Any reaction to PSA/PEA lectin was not found in surface epithelial cells and their apical cytoplasm in the pylorus region. A very strong reaction to AIA and DBA was noticed in a narrow area above the nucleus of glandular epithelial cells.

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Schumacher, U., Duku, M., Katoh, M., Jörns, J., Krause, W., 2004. Histochemical similarites of mucins produced by Brunner's glands and pyloric glands: Acomperative study. Anatomical Record, 278A, 540-550.
Machado-Santos, C., Pelli-Martins, A., AbiduFigueiredo, M., Brito-Gitirana, L., 2014. Histochemical and Immunohistochemical Analysis of The Stomach of Rhinella Icterica (Anura, Bufonidae). Journal of Histology, 8s, ID 872795.
Suprasert, A., Pongchairerk, U., Pongkent, P., Nishida, T., 1999. Lectin Histochemical Characterization of Glycoconjugates Present in Abomasum Epithelium of the Goat. Kasetsart Journal (Natural Science), 33, 234-242.
Rios-Martin, J., Diaz Cano, J., Rivera Hucto, F., 1993. Ultrastructural dsitribution of lectin binding sites on gastric superficial mucussecreting epithelial cells. Histochemistry, 99, 181-189.
Domeneghini, C.D., Arrighi, S., Radaelli, G., Bosi, G., Vegetti, A., 2005. Histochemical analysis of glycoconjugate secretion in the alimentary canal of Anguilla anguilla. Acta Histochemica, 106, 477-487.
Liquori, G.E., Mastrodonato, M., Zizza, S., Ferri, D., 2007. Glycoconjugate histochemistry of the digestive tract of Triturus carnifex (Amphibia, Caudata). Journal of Molecular Histology,38, 191-199.
Sommer, U., Rehn, B., Kressin, M., 2001. Light and electron microscopic investigation of the lectin-binding pattern in the oxyntic gland region of bovine abomasum. Annals of Anatomy, 183, 135-143.
Leknes, I.L., 2015. Mucin in epithelial cells in oesophagus and stomach of black tetra, Gymnocorymbus ternetzi (Characidae, Teleostei), Zoomorphology, 134, 269–277
Leknes, I.L., 2011. Histochemical studies on mucin-rich cells in the digestive tract of av teleost, the Buenos Aires tetra (Hyphessobrycon anisitsi). Acta Histochemica, 113, 353-357.
Pedini, V., Dall'Aglio, C., Parillo, F., Scocco, P., 2005. Glycoconjugate distribution in gastric fundic mucosa of Umbrina cirrosa L. revealed by lectin histochemistry. Journal of Fish Biology, 66, 222-229.
Desantis, S., Acone, F., Zizza, S., Deflorio, M., Fernandez, J.P., Sarasquete, C., De Metrio, G., 2009. Glycohistochemical study of the toadfish Halobatrachus didactylus (Scheider, 1801) stomach.Scıentıa Marına, 73, 3, 515-525.
Ito, M., Takata, K., Saito, S., Aoyagi, T., Hirano, H., 1985. Lectin-binding pattern in normal gastric mucosa. Histochemistry, 83, 189-193.
Sancar-Bas, S., Kaptan. E., Sengezer-Inceli, M., Sezen, A., Us, H., 2009. Glycoconjugate Histochemistry in the Fundic Stomach and Small Intestine of the Frog (Rana ridibunda). IUFS Journal of Biology, 68(2): 93-104.
Choi, B., Jo, U., Gil, Y.,2003. Glycocojugate of the gastric mucosa in cold tread chipmunks. Acta Histochemica, 105, 4, 309-317.
Suganuma, T., Tsuyama, S., Suzukı, S., Murata, F., 1984. Lectin-Peroxidase Reactivity in Rat Mucosa. Archive Histology and Cytology,47, 2, 197-207.
Diaz, A.O., Garcia, A.M., Goldemberg, A.L., 2008. Glycoconjugate in the mucosa of digestive tract of Cynoscion guatucupar: A histochemical study. Acta Histochemica, 110, 76-85.
Yamamoto, K., Qi, W., Yokoo, Y., Miyata, H., Udayanga, K.G., Kawano, J., Yokoyama, T., Hoshi, N., Kitagawa, 2010. Lectin histochemical detection of special sugars on the mucosal surfaces of the rat alimentary tract. The Journal of Veterinary Medical Science, 72, 9, 1119-1127.
Shimokawa, T., Doihara, T., Makara, M., Miyawaki, K., Nabeka, H., Wakisaka, H., Kobayashi, N., Matsuda, S., 2012. Lectin Binding Pattern of Gastric Mucosa of Pacific White-Sided Dolphin, Lagenorhynchus obliquidens Journal Vet. Med. Sci. 74(2), 155–160.
Scillitani, G.S., Zizza, G.E., Liquori, D., Ferri., 2007. Lectin histochemistry of gastrointestinal glycoconjugates in the greater horseshoe bat, Rhinolophus ferrumequinum. Acta Histochem, 109, 347-357.
Schwarz, F.P., Puri, K.D., Bhat, R.G., Surolia, A., 1993. Thermodynamics of Monosaccharide Binding to ConcanavalinA , Pea (Pisum sativum) Lectin, and Lentil (Lens culinaris) Lectin. The Journal of Biological Chemistry. 268, 11, 7668- 7677.
Liener I. E., Sharon N., Goldstein I. J., 1986. The Lectins – Properties, Functions and Applications in Biology and Medicine.
Dao-Thi, M.H., Rizkallah, P., Wyns, L., Poortmans, F., Loris, R., 1998. The quaternary structure of UEA-II, the chitobiose specific lectin from gorse. Acta Crystallogr, D54, 844-847.
Van Damme, E.J., Peumans, W.J., Barre, A., Rouge, P., 1998. Plant lectins: A composite of several distinct families of structurally and evolutionary related proteins with diverse biological roles. Critical Reviews in Plant Sciences, 17: 6, 575- 692.
Aypak, S.Ü., Uysal, H., 2010. Glikoproteinlerin Yapısı ve Fonksiyonları. Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi, 24, 2, 107-114.
Fukutomi, T., Itabashi, M., Tsuane, S., Yamamoto, H., Nanasawa, T. and Hiroto, T. 1989. Prognostic contributions of Helix pomatia and carcinoembryonic antigen using histochemical techniques in breast carcinoma. Jpn J Clin Oncol, 19, 127- 34.
Kawai, T., Suzuki, M., Torikata, C., Suzuki, Y., 1991. Expression of blood group-related antigens and Helix pomatia agglutinin in malignant pleural mesothelioma and pulmonary adenocarcinoma. Hum Pathol, 22, 118-24.
Narita, T., Numao, H., 1992. Lectin binding patterns in normal, metaplastic, and neoplastic gastiric mucosa. J Histochem Cytochem., 40: 681-687.
Elinger, A., Pavelka, M., 1992. Subdomains of the rough endoplasmic reticulum in colon goblet cells of rat: Lectin-cytochemical characterization. J Histochem Cytochem, 40, 919-930.
Seyrek, K., Bildik, A., 2001. Lektinler. Y.Y.Ü Veterinerlik Fakültesi Dergisi, 12 (1), 96-100.
Lehninger, A.L., Nelson, D.L., Cox, M.M., 2005. Lehninger Biyokimyanın Ilkeleri. (Kılıç, N., -ed.) Palme Yayıncılık, 1152 s. Ankara.
Sharon, N., Lis, H., 1977. Lectins: Their Chemistry and Application to Immunology. The Antigens., 4, 429-529.
Murnane, R.D., Ahern-Rindell, A.J. and Prieur, D.J. 1989. Lectin Histochemistry of an Ovine Lysosomal Storage Disease with Deficiencies of _-Galactosidase and ]- Neuraminidase. Amer J Pathol, 135, No. 4.
Özden, M., 2012. Anatomi ve Fizyoloji. 18. Baski, Ankara.
Eşrefoğlu, M., 2009. Özel Histoloji. Medipres Matbaacılık Yayıncılık, 328 s. Malatya.
Yörük, N.M., 2008. Sindirim Sistemi II: Sindirim Kanalı. Veteriner Özel Histoloji. (Özer, A., -ed), Nobel Yayın Dağıtım, 1. Baskı, 161-183s, Ankara.
Tanyolaç, A., 1999. Özel Histoloji. Yorum Basın Yayın Sanayi Ltd, 2.Baskı, 79s, Ankara.
Yaman, K., 1999. Fizyoloji. VIPAŞ A.Ş., 3. Baskı, 564s. Bursa.