Electrochemical sensing and fluorescence imaging of E. coli O157:H7 based on aptamerconjugated semiconducting nanoparticles

Aptamers are selective molecules against to various sizes of targets from small molecules to mammalian cells. Here, we reported QDs containing electrochemical aptasensor for the detection of E. coli O157:H7. The electrode surfaces were modified by cysteamine (CysN), which has amine and thiol groups, via self-assembled monolayer formation. The carboxyl-functionalized quantum dots (QD) and aptamers (Apt) were conjugated to cysteamine modified gold electrodes. Linear range for E. coli O157:H7 was from 1 to 102 CFU/mL after incubation on CysN/QD/Apt modified Au surfaces. QDs provide fluorescence surface, so that adhesion of cells was followed using fluorescence microscope. Adhered cells were also imaged by scanning electron microscopy. Finally, cell analysis was carried out in real samples.

Aptamer konjuge edilmiş yarı iletken nanoparçacıkları temel alan patojen bir mikroorganizmanın elektrokimyasal belirlenmesi ve floresans görüntülenmesi

Aptamerler, küçük moleküllerden memeli hücrelerine kadar çeşitli boyutlardaki hedeflere karşı seçici moleküllerdir. Burada, E. coli O157:H7 dedeksiyonu için QD içeren elektrokimyasal aptasensör rapor edilmiştir. Yüzeyler, amino ve tiyol grupları içeren sisteamin (CysN) ile kendiliğinden oluşan tek tabaka meydana gelişi ile modifiye edildi. Karboksil ile fonksiyonelleştirilen quantum noktalar (QD) ve aptamerler (Apt), sisteaminle modifiye edilmiş altın elektrotlara konjuge edildi. CysN/QD/Apt modifiye edilen Au yüzeylerde inkübasyon sorasında, E. coli O157:H7 için doğrusal aralık 1 to 102 CFU/mL idi. QD floresans yüzeyler sağlar, böylece hücrelerin adezyonu floresans mikroskop kullanılarak takip edildi. Adezyon sonrası hücreler aynı zamanda taramalı electron mikroskobu ile görüntülendi. Son olarak, hücre analizi gerçek örneklerde gerçekleştirildi.

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