Ökkeş YILMAZ, Ersin DEMİR, Halise SARIGÜL

Sulu Çam Çıra Ekstresinin Diyabet Oluşturulan Sıçanların Testis Dokusu Üzerindeki Etkisi

Bu çalışmanın temel amacı Tip 2 diyabetle indüklenen testis hasarında oksidatif stresin baskılanmasında sulu çam çıra ekstresinin olası etkilerini araştırmaktır. Çalışmada 49 adet 2,5 aylık erkek sıçanlar tercih edildi. Sıçanlar; kontrol, diyabet ve farklı dozlardan oluşan diyabet+çam çırası olmak üzere gruplara ayrıldı (D+Ç100, D+Ç200, D+Ç400). Kontrol grubu dışındaki sıçanlara 45 mg/kg streptozotozin (STZ) intraperitonal olarak verilerek Tip-2 diyabet oluşturuldu. Diyabet oluşumundan sonra sıçanların içme sularına farklı dozlarda (100 g/L, 200 g/L, 400 g/L) çam çıra ekstresi eklenerek suya serbest olarak erişimleri sağlandı. Deneysel uygulamalar 10 hafta sürdü. Kontrol grubu ile karşılaştırıldığında, diyabet grubunda MDA seviyesinin kayda değer düzeyde arttığı (p<0.001), total protein düzeyinin ise önemli düzeyde azaldığı (p<0.05) belirlendi. Diyabet grubu ile karşılaştırıldığında çıra suyu verilen gruplarda MDA düzeyinin önemli düzeyde azaldığı (p<0.001), protein seviyesinin ise önemli düzeyde yükseldiği (D+Ç100) (p<0.05) belirlendi. İncelenen diğer parametrelerde (α-tokoferol, palmitik, oleik, linoleik asit ve doza bağlı diğer parametreler) ise istatistiksel açıdan anlamlı değişiklikler tespit edildi (p<0.05, p<0.01, p<0.001). Sonuç olarak, sulu çam çıra ekstresinin oksidatif stres seviyesini azaltarak testiküler hasarı azaltmada potansiyel olarak yararlı olduğu gözlenmiştir.

Anahtar Kelimeler:

Çam çıra ekstresi, Diyabet; Oksidatif stres; Vitamin; Yağ asidi

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