FİGEN ESİN KAYHAN, Güllü KAYMAK, Harika Eylül Esmer DURUEL, Şeyma KIZILKAYA

Some oxidative stress parameters in heart tissue of Zebrafish (Danio rerio) caused by mancozeb

The potential toxic effects of mancozeb exposure on some antioxidant enzyme were investigated on heart tissue of zebrafish in this study. Zebrafish groups were exposed to different doses of mancozeb (Group A: 5 mgL-1 and Group B: 7.5 mgL-1) for 120 hours. In this study, catalase (CAT) activity, malondialdehyde (MDA) level and total protein (TP) level were determined with spectrophotometer. Our results showed that CAT activity was found 2.541±0.771 mg L-1 in A group and 2.011±0.201 mg L-1 in B group in this study. CAT and MDA activity levels decreased in the experiment group according to control group. MDA levels were found 0.025±0.003 mg L-1 in A group and 0.025±0.003 mg L-1 in B group. TP levels were found 9.75±1.51 mg L-1 in A group and 10.18±0.32 mg L-1 in B group. TP levels increased in the all experiment groups according to control group. We observed that the changes in the CAT activity and MDA levels were time and as well as mancozeb dose dependent. As a result, mancozeb is a very toxic substance for zebrafish and other aquatic organisms.

Zebrabalığı (Danio rerio) kalp dokusunda mancozeb’in neden olduğu bazı oksidatif stres parametreleri

Bu çalışmada, zebra balığı kalp dokusunda mancozeb’in bazı antioksidan enzimler üzerindeki potansiyel toksik etkileri araştırılmıştır. Zebra balığı grupları, 120 saat boyunca farklı dozlarda mancozeb (Grup A: 5 mg L-1 ve Grup B: 7,5 mg L-1) maruz bırakıldı. Bu çalışmada, katalaz (CAT) aktivitesi, malondialdehit (MDA) ve total protein (TP) seviyeleri spektrofotometre ile belirlenmiştir. Elde ettiğimiz sonuçlar CAT aktivitesinin ve MDA seviyesinin tüm deney gruplarında azaldığını gösterdi. Bu çalışmada CAT aktivitesi A grubunda 2,541±0,771 mg L-1, B grubunda ise 2,011±0,201 mg L-1 olarak bulunmuştur. CAT ve MDA aktiviteleri tüm deney gruplarında kontrol grubuna göre azalmıştır. MDA seviyeleri A grubunda 0,025±0,003 mg L-1 ve B grubunda 0,025±0,003 mg L-1 olarak bulunmuştur. TP seviyeleri A grubunda 9,75±1,51 mg L-1, B grubunda ise 10,18±0,32 mg L-1 olarak bulunmuştur. Deney grubunda TP düzeyi kontrol grubuna göre artmıştır. CAT aktivitesi ve MDA seviyelerindeki değişikliklerin deney süresine ve mancozeb dozuna bağlı olduğu belirlenmiştir. Sonuç olarak; mancozeb zebra balığı ve diğer sucul organizmalar için oldukça toksik bir maddedir.

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