Oxidative stress and antioxidant defenses after starvation in Oncorhynchus mykiss (Walbaum, 1792) larvae reached free swimming stage

Oxidative stress and antioxidant enzyme activities after starvation of Oncorhynchus mykiss larvae were studied. Just after yolk-sac absorption, free-swimming larvae were maintained under starved condition for a period of 21 days. Sampling of fish were carried out every week and their whole bodies were used for the analysis of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSHpx), glutathione reductase (GR), glutathione S-transferase (GST) and also analysis of malondialdehyde (MDA) and glutathione (GSH). Antioxidant enzymes activities showed that GST and CAT had the highest activity, whilts SOD and GSHpx had the lowest activity of all the enzymes assayed in 14 day free swimming starved larvae. SOD activity were reached to the peak in free swimming larvae before the appearance of higher level of MDA in 7 day starved larvae. The level of MDA was significantly higher in 7 day starved larvae and significantly lower in 14 day starved larvae. ΣPUFA and Σn-3 fatty acid have the highest level in 7 day starved larvae and lowest level in 14 day starved larvae. After the free swimming larvae, there was a significant decrease in vitamin E (α-tocopherol and δ-tocopherol) level in all starved larvae.

Serbest yüzme safhasına ulaşan Oncorhynchus mykiss (Walbaum, 1792) yavrularında açlık sonrası oksidatif stres ve antioksidan savunmalar

Bu çalışmada Oncorhynchus mykiss larvalarının açlıktan sonra oksidatif stres ve antioksidan enzim aktiviteleri çalışılmıştır. Yumurta kesesinin emilimiyle birlikte serbest yüzmeye geçen larvalar 21 gün boyunca aç bırakılmıştır. Balık örnekleri her hafta alınmış ve bütün vücutları, süperoksit dismutaz (SOD), katalaz (CAT), glutatyon peroksidaz (GSHpx), glutatyon redüktaz (GR), glutatyon S-transferaz (GST) analizi ve ayrıca malondialdehit (MDA) ve glutatyon (GSH) analizinde kullanılmıştır. 14 gün aç bırakılmış serbest yüzen yavrularda, analizi yapılan bütün antioksidan enzim aktiviteleri içerisinde, GST ve CAT en yüksek aktiviteye sahipken, SOD and GSHpx en düşük aktiviteye sahip olmuştur. 7 gün aç bırakılan larvalarda MDA’nın yüksek düzeyi ortaya çıkmadan önce SOD aktivitesi serbest yüzen yavrularda zirveye ulaşmıştır. MDA düzeyi 7 gün aç bırakılan larvalarda belirgin olarak daha yüksek ve 14 gün aç bırakılan larvalarda belirgin olarak daha düşük bulunmuştur. ΣPUFA ve Σn-3 yağ asidi, 7 gün aç bırakılan larvalarda en yüksek, 14 gün aç bırakılan larvalarda ise en düşük seviyeye sahip olmuştur. Serbest yüzen larvalardan sonra, aç bırakılan larvaların hepsinde E vitamini (α-tokoferol ve δ- tokoferol) seviyesinde belirgin bir azalma olmuştur.

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