Nannochloropsis oculato (Eustigmatophyceae)'nın besin olarak Melicertus kerathurus (Forksal, 1775) larvaları'nda kullanılması

Nannochloropsis oculata, eicosapentaenoic asit EPA, 20:5(n-3) ve arachidonic asit ARA, 20:4(n-6) içeriğince zengin besin değerinden dolayı bir çok deniz balıkları kuluçkahanesinde larva yetiştiriciliğinde (rotifer üretiminde ve larva tanklarında) canlı mikro- alg olarak yaygın şekilde kullanılmaktadır. Bununla birlikte, N. oculata karides larva beslenmesinde kullanılmamaktadır ve bu konu ile ilgili çalışmalar çok sınırlıdır. Bu çalışmadaki amaç N. oculata’nın Melicertus kerathurus larvalarının beslenmesinde tek başına besin olarak uygun olup olmayacağını karides kuluçkahanelerinde yaygın olarak kullanılan Chaetoceros calcitrans türü ile karşılaştırarak değerlendirmektir. Beş farklı (100, 150, 200, 250 ve 300 x 103 hücre/ml) yoğunlukta beslenen M. kerathurus larvalarının gelişim indeksleri ve yaşama oranları belirlenmiştir. Denemeler kontrol besini C. calcitrans ile beslenen larvaların %50’sinden fazlasının Myisis 1 olduğunda sonlandırılmıştır. Yaşama oranı kontrol grubunda %74.6 olarak gerçekleşmiş deneme gruplarında %27.3–40.6 arasında kaydedilmiştir. N. oculata ile beslenen larvaların gelişimlerinde C. calcitrans ile beslenenlere oranla kayda değer bir gerileme görülmüştür. Larval gelişim analizlerinde C. calcitrans ile beslenen larvaların ortalama gelişim indeksleri 3.7 ile Mysis 1’de sonuçlanırken deneme grubunda 2.0–2.3 arasında değişmiştir. Deneme gruplarında beslenme yoğunlukları ne olursa olsun yaşama oranları arasında fark bulunmazken (P > 0.05), 200 ve 250 x 103 hücre/ml yoğunlukta beslenen larvaların gelişim indeksleri 100, 150 ve 300 hücre/ml yoğunlukta beslenen larvalarınkinden daha büyük (P < 0.05) bulunmuştur.

Utilization of Nannochloropsis oculata (Eustigmatophyceae) as diet on Melicertus kerathurus (Foksal, 1775) larvae.

Utilization of Nannochloropsis oculata (Eustigmatophyceae) as diet on Melicertus kerathurus (Forskal, 1775) larvae. Nannochloropsis oculata is widely used as live microalgae for larval rearing (for rotifer production and into larval tanks) in many marine fish hatcheries because of its high nutritional value on eicosapentaenoic acid EPA, 20:5(n-3) and arachidonic acid ARA, 20:4(n-6). However, N. oculata has not been used as shrimp larvae feed and reports them to shrimp larvae are very scarce. The aim of the present study was to evaluate whether N. oculata could be used as monoalgal diet for Melicertus kerathurus larvae, compared to Chaetoceros calcitrans commonly used in shrimp hatcheries. Larval development index and survival rate of M. kerathurus larvae fed five different (100, 150, 200, 250, and 300 x 103 cell/ml) densities of N. oculata were estimated. The experiments were stopped when ≥ 50% of the larvae fed the control diet of C. calcitrans metamorphosed to Mysis 1. Survival rates were resulted as 74.6% in control group fed C. calcitrans and recorded between 27.3-40.6% in experimental groups. Larvae fed on N. oculata were considerably delayed in development as compared to those fed on C. calcitrans. In larval development analysis, feeding of C. calcitrans to Mysis 1 resulted in average development index 3.7, but in experimental groups was ranged from 2.0 to 2.3. In experimental group, although there was no difference (P > 0.05) in survival rate of larvae, regardless of their feeding density, development index of larvae fed 200 and 250 x 103 of N. oculata densities was higher (P < 0.05) than those fed 100, 150 and 300 x 103 densities.

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