Haematococcus pluvialis Flatow (Chlorophyceae)' un farklı ışık şiddetlerinde vejetatif büyüme özellikleri

Haematococcus pluvialis'm vejetatif kültürlerinde, optimal ışık şiddeti aralığı ve büyüme performanslarının tespiti için 50,100, 200, 400 ve 600 $mu$ mol foton $m^{-2}sn^{-1}$ 'lik beş farklı ışık şiddeti uygulandı. Deneme gruplarında kuru ağırlık ve toplam karaten miktarları artan ışık şiddetleri ile doğru orantılı olarak artarken, toplam klorofil miktarı 200 $mu$ mol foton $m^{-2}sn^{-1}$'lik aydınlatmaya kadar arttı (2.21 mg $L^{-1}$ ) ve bunun üzerindeki aydınlatma şiddetlerinde herhangi bir değişim görülmedi (ortalama 2.35 mg $L^{-1}$ ). Hücre sayısı tüm gruplarda karanlık periyoda denk gelen 15. saatte ortalama 3.45 x $10^4$ hücre $ml^{-1}$'den 18. saatte 5.66 x $10^4$ hücre $ml^{-1}$ 'ye arttı. Sonuç olarak, 200 $mu$ mol foton $m^{-2}sn^{-1}$ 'lik ışık şiddetinin üzerindeki aydınlatmalarda astaksantin birikiminin tetiklendiği ve hücre sayısının azaldığı görüldü (50,100, 200, 400 ve 600 $mu$ mol foton $m^{-2}sn^{-1}$ 'lik ışık şiddetleri için sırasıyla 12.73 x $10^4$ ,16.10 x $10^4$ , 19.73 x $10^4$ , 15.90 x $10^4$ ve 12,89 x $10^4$ hücre $ml^{-1}$ ). Beş farklı ışık şiddetinin uygulandığı denemede hücrelerin vejetatif safhada kültür edilebilmesi için optimal ışık şiddeti aralığı 50 - 200 $mu$ mol foton $m^{-2}sn^{-1}$ olarak bulundu ve en iyi büyüme 200 $mu$ mol foton $m^{-2}sn^{-1}$ 'lik ışık şiddetinde gerçekleşti.

Anahtar Kelimeler:

ışık, pigmentasyon, Chlorophyta, astaksantin

Vegatative growth characteristics of Haematococcus pluvialis Flotow at different light intensities

Five different light intensities of 50,100, 200, 400 and 600 $mu$ mol photon $m^{-2}sn^{-1}$ were applied on the vegetative cultures of Haematococcus pluvialis to determine the optimal light intensity, and the growth performance. While dry weight and total carotenoid amounts increased in parallel to the light intensities, total chlorophyll amount increased up to the irradiance of 200 $mu$ mol photon $m^{-2}sn^{-1}$ (2.21 mg $L^{-1}$ ), and no change was observed in the higher illuminations (mean value of 2.35 mg $L^{-1}$ ). Interestingly, cell count increased in all the groups from the mean value of 3.45 x $10^4$ cells ml1 on the 15th hour to 5.66 x $10^4$ cells $ml^{-1}$ on the 18th hour, which coincided with the dark period. In conclusion, astaxanthin accumulation was triggered and cell number was decreased above the illuminations of 200 $mu$ mol photon $m^{-2}sn^{-1}$ (12.73 x $10^4$ , 16.10 x $10^4$ ,19.73 x $10^4$ ,15.90 x $10^4$ and 12,89 x $10^4$ cells $ml^{-1}$ for 50,100,200,400 and 600 $mu$ mol photon $m^{-2}sn^{-1}$ , respectively). In the experiment applied five different illuminations, optimal light intensity range for the vegetative stage cultivation of the cells was found to be 50 - 200 $mu$ mol photon $m^{-2}sn^{-1}$ and the best growth was in the light intensity of 200 $mu$ mol photon $m^{-2}sn^{-1}$ .

Keywords:

light, pigmentation, Chlorophyta, astaxanthin,

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