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Çamaltı Tuzlasının su örneğinden izole edilen Planococcus dechangensis’in karakterizasyonu

Bu çalışmada, MHDS3 suşu Çamaltı Tuzlasının su örneğinden izole edilmiştir ve geleneksel ve moleküler yöntemlerle tanımlanmıştır. 16S rRNA gen dizi analizleri bu suşun Planococcus dechangensis türü olduğunu göstermiştir. Gram pozitif, kok formunda, hareketsiz, aerobik, katalaz pozitif, oksidaz negatiftir ve sarı-turuncu koloni pigmentasyonu göstermiştir. Bu suş sitrat kullanımı, triptofanda indol üretimi, Voges-Proskauer ve metil kırmızısı testlerinde negatif sonuç vermiştir. Bu izolat 10-45°C’de (optimum 35°C), pH 6-8’de (optimum pH 7) ve %3-20 NaCl konsantrasyonunda (optimum %10 NaCl) gelişebilmektedir. 4°C, 10°C, 50°C’de, tuzsuz ortamda, %0.5, %25, %30 toplam tuz oranında, pH 4-5 ve pH 9-12’de gelişme göstermemiştir. Glukoz, riboz, fruktoz, sukroz, maltoz bu izolat tarafından karbon kaynağı olarak kullanılmıştır. Hayvan derisinin yapısında bulunan L-lizin, L-arjinin, L-sistein, L-alanin, L-tirozin, L-histidin gibi farklı amino asitler bu izolat tarafından da kullanılmıştır. Tuz üretimi aşamasında, bu bakteri gıda ve deri endüstrisinde kullanılmak üzere üretilen tuzu kontamine edebilir. Ilımlı halofil bakterilerin zararlı etkileri uygun antimikrobiyal uygulamalar ile engellenmelidir.

Characterization of Planococcus dechangensis isolated from a water sample of Çamaltı Saltern

In the present study, strain MHDS3 was isolated from a water sample of Çamaltı Saltern and identified using conventional and molecular methods. 16S rRNA gene sequence analyses showed that the strain MHDS3 belonged to Planococcus dechangensis species. It gave a positive result in the Gram staining test. The cells were coccus, non-motile, aerobic, catalase positive, oxidase negative and the colony pigmentation was yellow-orange. It showed negative results for citrate utilization, indole production from tryptophane, Voges-Proskauer and methyl red. This isolate was able to grow at 10-45°C (optimally 35°C), pH 6-8 (optimally pH 7) and 3-20% NaCl (optimally 10% NaCl). It was not able to grow at 4°C, 10°C, 50°C, salt-free, 0.5%, 25%, %30 total salt, pH 4-5, and pH 9-12. Glucose, ribose, fructose, sucrose, maltose were used by the test isolate as carbon sources. Different amino acids found in the structure of animal hide such as L-lysine, L-arginine, L-cysteine, L-alanine, L-tyrosine, L-histidine were also utilized by the bacterium. During the salt production process, this bacterium may contaminate the salt which is used in the food and leather industries. The activities of harmful moderately halophilic bacteria should be prevented by effective antimicrobial applications.

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