Deniz İzlen ÇİFÇİ, Rıza ATAV, Yalçın GÜNEŞ, Elçin GÜNEŞ

Effect of parameters on removal of RB19 dye by laccase enzyme and kinetic evaluation

Dye decolorization of RB19 using commercial laccase (Cerrena unicolor) enzyme was evaluated in this study. The effect of pH, laccase concentration, temperature, metal salt, carbon and nitrogen sources and solvents on the removal of RB19 dye were examined in detail. Highest RB19 removal with laccase (Cerrena unicolor) was obtained at pH 6, 40°C, 0.3 g/L enzyme concentration and 200 mg/L initial dye concentration. According to the Michaelis–Menten kinetics, the highest Vmax was 6.61 mg/L.min at these conditions. 0.25 mM of Cu2+ or Mn2+ enhanced the decolorization of RB19 but further increase in metal concentration decreased the removal of RB19. In the presence of carbon source such as glucose, starch and lactose, removal of RB19 by laccase was not significantly affected. However, in the presence of nitrogen sources such as urea and NH4Cl, the removal of RB19 by laccase was significantly reduced.

Keywords:

Cerrena unicolor enzymatic treatment, kinetic, laccase, Reactive Blue 19.,

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Sigma Mühendislik ve Fen Bilimleri Dergisi-Cover

ISSN: 1304-7191
Yayın Aralığı: Yılda 4 Sayı
Yayıncı: Yıldız Teknik Üniversitesi

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