ACANTHUS HIRSUTUS ‘UN ASETON ÖZÜTÜNÜN ANTİOKSİDAN ÖZELLİKLERİNİN ARAŞTIRILMASI
Acanthus hirsutus Anadolu geleneksel halk hekimliğinde çeşitli hastalıkların tedavisinde kullanılmaktadır. Bu çalışmada A. hirsutus’un aseton ekstraktının antioksidan özellikleri; toplam antioksidan, serbest radikal süpürme etkinliği (DPPH testi), β-karoten/linoleik asit metodu, demir ve bakır indirgeme güçlerini içeren farklı kimyasal test sistemleri kullanılarak değerlendirilmiştir. Toplam fenolik ve flavonoid içerikler belirlenmiştir. Toplam fenolik ve flavonoid içerik sırasıyla 51.39 mgGAE/g ve 18.60 mgRE/g olarak tespit edilmiştir. DPPH yönteminde 1mg/ml konsantrasyonda %47.87 etkinlik gözlenmiştir. β-karoten/linoleik asit test sisteminde, 1 mg/ml konsantrasyon linoleik asit oksidasyonu %66.83 oranında inhibe edilmiştir. Demir ve bakır indirgeme güçleri ise konsantrasyona bağlı bir durum sergilemektedir. Bu sonuçlar A. hirsutus’un doğal antioksidanların bir kaynağı olarak gıda ve farmakoloji endüstrilerinde kullanılabileceğini göstermektedir.
Anahtar Kelimeler:
Acanthus hirsutus, Antioksidan, Fenolik içerik, Serbest radikal, Türkiye
INVESTIGATION OF ANTIOXIDANT PROPERTIES OF ACETONE EXTRACT OF ACANTHUS HIRSUTUS
Acanthus hirsutus is used in Anatolian folk medicine for the treatment of various ailments. In the present study, the antioxidant properties of acetone extract obtained from A. hirsutus was evaluated by different chemical assays, including total antioxidant, free radical scavenging activity (DPPH method), β-carotene/linoleic acid test system, ferric and cupric reducing power. Total phenolic and flavonoid content were also determined. Total phenolic and flavonoid content were detected as 51.39 mgGAE/g and 18.60 mgRE/g, respectively. The free radical scavenging activity was observed as 47.87% at 1 mg/ml concentration in DPPH method. In β- carotene/linoleic acid system at 1 mg/ml concentration, the extract exhibited 66.83% inhibition against linoleic acid oxidation. Moreover, the ferric and cupric reducing powers were concentration-dependent manner. The results indicated that A. hirsutus can be exploited as a source of natural antioxidants in food and pharmacological industry
Keywords:
Acanthus hirsutus, Antioxidant, Phenolic content, Free radical, Turkey,
___
- Aktumsek A, Zengin, G, Guler, G.Ö, Cakmak, Y.S, Duran A (2013). Antioxidant potentials and anticholinesterase activities of methanolic and aqueous extracts of three endemic Centaurea L. species, Food and Chemical Toxicology 55, 290-296.
- Apak R, Guclu, K, Ozyurek, M, Karademir, S.E, Ercag E. (2006). The cupric ion reducing antioxidant capacity and polyphenolic content of some herbal teas, International Journal of Food Sciences and Nutrition 57(5/6), 292-304.
- Arvouet-Grand, A, Vennat, B, Pourrat, A, Legret P (1994). Standardisation d’un extrait de propolis et identification des principaux constituants, Journal de Pharmacie de Belgique 49, 462–468.
- Barış, Ö, Güllüce, M, Şahin, F, Özer, H, Kılıç, H, Özkan, H, Sökmen, M, Özbek T (2006). Biological activities of the essential oil and methanol extract of Achillea biebersteinii Afan. (Asteraceae), Turkish Journal of Biology 30, 65-73.
- Baytop T (1999). Türkiye’de Bitkiler İle Tedavi, Nobel Tıp Kitabevi Yayınları, İstanbul.
- Bhattacharya B, Johri B.M (1998). Flowering Plants: Taxonomy and Phylogeny. Berlin.
- Cakmak Y.S, Aktumsek, A, Duran A (2012). Studies on antioxidant activity, volatile compound and fatty acid composition of different parts of Glycyrrhiza echinata L, EXCLI Journal 11, 178-187.
- Demiriz Y (1984). Acanthus:Türkiye’nin arkeoloji ve sanat tarihi terminolojisine yanlış adla girmiş bir bitki motifi,Ege üniversitesi,Edebiyat Fakültesi,Arkeoloji-Sanat Tarihi Dergisi 3,19-24.
- Halliwell B, Gutterıdge JMC (1999). Free radicals in Biology and medicine (3rd ed.), Oxford University Press.
- Hokputsa S, Harding, S.E, Inngjerdingen, K, Jumel, K, Michaelsen, T.E, Heinze, T, Koschella, A, Paulsen BS (2004). Bioactive Polysaccharides from the Stems of the Thai Medicinal Plant Acanthus ebracteatus: Their Chemical and Physical Features, Carbohydrate Research 339, 753-762.
- Kanchanapoom T, Noiarsa, P, Otsuka, H, Ruchirawat S (2006). Chemical Constituents of Acanthus volubilis Wall., Biochemical Systematics and Ecology 34(5), 442-445.
- Kumaran A, Karunakaran RJ (2007). In vitro antioxidant activities of methanol extracts of five Phyllanthus species from India, LWT-Food Science and Technology 40, 344-352.
- Matés J.M, Pérez-Gómez, C, Núnez de Castro, I(1999). Antioxidant enzymes and human diseases. Clinical Biochemistry 32, 595–603.
- Namıkı M (1990). Antioxidants/antimutagens in food. Critical Reviews in Food Science and Nutrition 29, 273300.
- Ozturk M, Aydogmus-Ozturk, F, Duru, M.E, Topcu G (2007). Antioxidant activity of stem and root extracts of Rhubarb (Rheum ribes): An edible medicinal plant. 103, 623-630.
- Perkins MJ (1995). Radical Chemistry, Ellis Horwood, London,
- Prieto P, Pineda, M, Aguilar M (1999). Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphor molybdenum complex: Specific application to the determination of vitamin E, Analytical Biochemistry, 269, 337–341.
- Sarikurkcu C, Arisoy, K, Tepe, B, Cakir, A, Abali, G, Mete E (2009). Studies on the antioxidant activity of essential oil and different solvent extracts of Vitex agnus castus L. fruits from Turkey, Food and Chemical Toxicology, 47, 2479-2483.
- Savran A, Bağcı, Y, Kargıoğlu M (2002) Gemerek (Sivas) ve Çevresindeki Bazı bitkilerin Yerel adları ve Etnobotanik , Afyon Kocatepe Üniversitesi Fen Bilimleri Dergisi,8(1),
- Singleton VL, Rossi, JA (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16, 144-158.
- Sokmen A, Gulluce, M, Akpulat, H.A, Daferera, D, Tepe, B, Polissiou, M, Sokmen, M, Sahin F (2004). The in vitro antimicrobial and antioxidant activities of the essential oils and methanol extracts of endemic Thymus spathulifolius, Food Control 15, 627-634.
- Tepe B, Sokmen, M, Akpulat, A.H, Yumrutas, O, Sokmen A (2006). Screening of antioxidative properties of the methanolic extracts of Pelargonium endlicherianum Fenzl., Verbascum wiedemannianum Fisch.&Mey., Sideritis libanotica Labill. subsp. linearis (Bentham) Borm., Centaurea mucronifera DC. and Hieracium cappadocicum Freyn from Turkish flora, Food Chemistry 98, 9-13.
- Wei YH, Pang CY (2005). The Role of Mitocondria in human aging process. Biotech International 17, 8-13.
- Başlangıç: 1981
- Yayıncı: Selçuk Üniversitesi