In this study examined that PTO power and torque values, tractor hourly fuel consumption and tractor specific fuel consumption of three tractors with different nominal loads driven with a 540 and 540E min-1 power take-off (PTO) and a centrifugal pump with a reduction gear. A 150 mm intake line with 1/4 transmission system driven with a PTO, horizontal shaft snail type centrifugal gear pump with 125 mm nominal diameter with outlet line was used in the study. The pump was driven by three different power tractors (New Holland TD65D, TD90D and TD110D) under laboratory conditions with 540 and 540E PTO options. The pump was operated at constant shaft speed (2160 min-1) during all tests. PTO torque, PTO power, fuel consumption and specific fuel consumption parameters were determined for both PTO applications in the experiments. The pump operations in all three tractor revealed that there was no change in PTO torque values as a result of experiments carried out with 540 and 540E rpm while the values fuel consumption in the 540E rpm application (14% for TD110D tractor, 34% for the TD90D tractor, 11% for the TD65D tractor) and specific fuel consumption (17% for the TD110D tractor, 36% for the TD90D tractor, 13% for the TD65D tractor) were less than the values in the 540 rpm application. This is the result of the application of different engine speeds for the two PTOs. Taking all parameters into account, it has been concluded that the 540E application provides certain advantages in terms of fuel consumption for many tools and machines operated with PTO compared to the 540 application. According to this result, the 540E application is proposed as an important alternative to the 540 application for tools and machines of similar capacity and specifications used in this study.