Aronia (Aronia melanocarpa L.), known as both small fruit and ornamentally valuable plant, could be propagated by seeds although this method is not recommended. The most easy method to propagate it is using in vitro techniques by following micro propagation technology. In this study semi-hardwood buds from aronia plant the explant length 1cm and, each of explant contains one lateral bud figure 2 (explant –a). Based in this study to reproduce aronia in in vitro by tissue culture technology and by using micro propagation was obtained on suitable culture medium, where the highest shoot length (14.60 mm) achieved at MS basal medium which combination of different concentrations of growth regulators (1.0 mg l-1 BA +0.02 mg l-1 IAA 0.1 mg l-1 GA3) at treatment 8. The highest shoot number (64 unit) were obtained at MS basal medium containing a combination of growth regulators at different concentrations (2.0 mg l-1 BA +0.01 mg l-1 IAA+0.1 mg l-1 GA3) at treatment 6. The highest callus rate achieved at treatments (3, 4, 10) respectively, all the procedures in in vitro was obtained within 4 weeks, and so this include rooting process where the highest root number (9.5 unit) at MS control at 0.0 mg l-1 concentration of IBA. The most suitable MS basal medium for root length containing 1.0 mg l-1 concentration of IBA in length (18 mm) and the highest plant length (33 mm) at 2.0 mg l-1 concentration of IBA. Concerninig results of infection, two subcultures considered best subculture compared to other subcultures from where number of death plants and infected plants particularly free of bacterial infections, where the percentage of healthy plants (99%).