This study was conducted at Selçuk University, Faculty of Agriculture, Department of Horticulture, Research and Application Orchard. In the study, the effects of different bacterial strains on yield, fruit quality, plant growth, and nutrition issues in Jumbo and Chester blackberry varieties in calcareous soil conditions were determined. As a result of the measurements and analyzes, it was determined that bacterial applications are effective on plant growth, yield, and fruit quality criteria. Shoot length values were found longer in the Chester variety, while the Jumbo variety had higher values in the number of shoots per plant. There was no statistically significant difference in terms of yield per decare of varieties. When the efficiency is examined in terms of applications, 637Ca application in Chester variety and 637Ca + SY 55 application in Jumbo variety have the highest efficiency values. With the data obtained, it was determined that bacterial applications made positive contributions to blackberry plants grown in calcareous soil conditions.