Fatty acid composition of Acanthoscelides obtectus adults which were exposed to low temperature (+4°C) for 10, 20 and 30 days were investigated. A. obtectus were reared in condition of 28 ± 2°C and 65 ± 5% relative humidity and dark until they were adult and beans were used as feed. The fatty acid compositions of triacylglycerol that were extracted from whole body of adult A. obtectus were analyzed using gas chromatography. It was determined that total fatty acid compositions of adults were composed of C12:0-C21:0 fatty acids. The major components determined in samples were oleic acid (C18: 1ω9), palmitic acid (C16: 0) and linoleic acid (C18: 2ω6). It was showed that oleic acid (59.56-61.74%), palmitic acid (16.36-18.02), linoleic acid (7.21-8.38%) and stearic acid (6.29-7.34%) constituted the major part of fatty acid compositions of adult. The results obtained from fatty acid composition analyses revealed that the decrease in percentage of total saturated fatty acid (SFA) was accompanied by increase in percentage of total monounsaturated (MUFA) fatty acids at low temperature.