Zeliha AYTEKİN, Ayşe TORAMAN, Kübra KARAÇAM

Sıçan Periodontitis Modelinde Lokal C Vitamini Uygulamasının İnflamatuar Yanıt ve Periodontal Doku Yıkımı Üzerine Etkilerinin Araştırılması

Amaç: Bu çalışmanın amacı deneysel periodontitisli ratlarda lokal C vitamini uygulamasının inflamatuar yanıt ve periodontal doku yıkımı üzerine olan etkisini değerlendirmektir. Gereç ve Yöntemler: Çalışmada her grupta 7 sıçan olmak üzere toplam 21 hayvan kullanıldı: 1-Ligatür bağlanmayan (NL) 2-deneysel periodontitis (EP) ve 3-lokal vitamin C (VtC) grupları. EP ve VtC grubu sıçanlarda deneysel periodontitis oluşturuldu. Ligatürler 11 gün sonra ligatür çıkarıldı. EP ve VtC grubu sıçanların vestibül sulkusuna sırasıyla 50 µL salin solüsyonu ve C vitamini lokal olarak enjekte edildi. Ratlar sakrifiye edildikten sonra kan ve dişeti dokusu örnekleri alındı. TNF-α seviyesi serumda biyokimyasal olarak, 8-OHdG ve MMP-8 düzeyleri ise dişeti dokusunda immünohistokimyasal olarak analiz edildi. Mandibula örnekleri kemik yıkımı ve ataşman kaybı açısından histolojik olarak analiz edildi. Bulgular: 8-OHdG ve MMP-8 immünopozitif hücre yoğunluğunun Vtc grubunda EP grubuna göre istatistiksel olarak anlamlı derecede daha düşük olduğu bulundu. C vitamini uygulaması, EP grubuna kıyasla VtC grubunda ataşman kaybını ve kemik kaybını önemli ölçüde azalttı. Ancak EP grubuna göre VtC grubunda serum TNF-α düzeyinde anlamlı bir fark yoktu. Sonuç: Lokal C vitamini uygulaması, C vitamininin antioksidan aktivitesi ile inflamatuar yanıtı destekleyerek ve potansiyel immünomodülatör etkisi ile inflamasyonun çözülmesine katkıda bulunarak inflamasyona bağlı kemik yıkımını azaltabilir. C vitamini periodontitis tedavisinde kullanılabilecek terapötik bir ajan olabilir.

Anahtar Kelimeler:

antioksidanlar, Deneysel periodontitis, oksidatif stres, Vitamin C

Investigation of Effects of Local Vitamin C Application on Inflammatory Response and Periodontal Tissue Destruction in Rat Periodontitis Model

Background: The present study aims to evaluate the effect of local vitamin C application on inflammatory response and periodontal tissue destruction in rats with experimental periodontitis. Methods: A total of 21 animals, 7 rats in each group, were used in the study: 1- Non-ligation (NL), 2-experimental periodontitis (EP), and 3- local vitamin C (VtC) groups. Experimental periodontitis was induced in EP and VtC group rats. After 11 days, the ligature was removed. 50 mL of saline solution and vitamin C were injected locally into the vestibule sulcus of the EP and VtC group rats, respectively. After the rats were sacrificed, blood and gingival tissue samples were taken. TNF-α levels were analyzed biochemically in serum and 8-OHdG and MMP-8 were analyzed immunohistochemically in gingival tissue. The mandibular specimens were histologically evaluated for bone destruction and attachment loss. Results: The numeric density of 8-OHdG and MMP-8 immunopositive cells were found to be statistically significantly lower in the Vtc group than in the EP group. Vitamin C administration significantly reduced attachment loss and bone loss in the VtC group as compared to the EP group. However, there was no significant difference in serum TNF-α level in the VtC group compared to the EP group. Conclusions: Local application of vitamin C may reduce inflammation-related bone destruction by supporting the inflammatory response with the antioxidant activity of vitamin C and contributing to the resolution of inflammation with its potential immunomodulatory effect. Vitamin C may be a therapeutic agent that can be used in the treatment of periodontitis.

Keywords:

antioxidants, Experimental periodontitis, oxidative stress, vitamin C,

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