Meltem MERT EREN, Günçe OZAN, Cansu VATANSEVER

Farklı Propolis Damlalarının Oral Patojenlere KarşıAntimikrobiyal Etkinliğinin Değerlendirilmesi: Bir İn Vitro Çalışma

Amaç: Bu in vitro çalışmanın amacı, Türkiye'de kolaylıkla bulunançeşitli propolis damlalarının iki oral patojene (Streptococcusmutans ve Enterococcus faecalis) karşı antimikrobiyaletkinliklerinin değerlendirilmesidir. Gereç ve Yöntemler: Çalışmaya yedi farklı propolis damlası (1'den7'ye gruplar: Umay Herbal Organic Propolis, Bee'o Up [%15],Propoli EVSP, Brazilian Green Liquid Propolis, Eğriçayır Propolis,Bee'o Up [%30], Biostore Propolis) dahil edildi. Bakteriler 100 µl,her bir propolis örneği ise10 µl olacak şekilde yayma ekim tekniğiile Brain-Heart Infusion agar besiyerine ekildi. Petri kutuları bakteriüremesi için 24 saat inkübasyona bırakıldı ve sonuçta oluşan zonçapları ölçüldü. Her deney grubu fotoğraflandı. İstatistiksel analizMann Whitney-U testi kullanılarak yapıldı. Bulgular: Su bazlı propolis (Grup 1) iki oral patojene karşı dainhibisyon bölgesi oluşturamamıştır. Alkol bazlı propolislerden ise,yalnızca 4. ve 5. gruplar E. faecalis' e karşı etkinlikgösterememiştir. S. mutanslara karşı en yüksek antimikrobiyal etki5. grup'ta görülürken, aynı grup E. faecalis üzerinde herhangi biretki gösterememiştir. 6. grup, E. faecalis üzerinde diğer propolisdamlalarından anlamlı olarak daha etkili bulunmuştur (p

Antimicrobial Effect of Propolis Drops On Oral Pathogens: AnIn Vitro Study

Background: The aim of this in vitro study was to evaluate theantimicrobial effectiveness of various propolis drops found easilyin Turkey against two oral pathogens; Streptococcus mutans andEnterococcus faecalis. Methods: Seven different propolis drops (Group 1 to 7: UmayHerbal Organic Propolis, Bee'o Up [15%], Propoli EVSP, BrazilianGreen Liquid Propolis, Eğriçayır Propolis, Bee'o Up [30%],Biostore Propolis) were included in the study. Antimicrobial effectof drops was investigated by the agar plate diffusion method. Petridishes were prepared with Brain-Heart Infusion broth. 100 µl ofmicroorganisms and 10 µl of each propolis were planted to thesepetri dishes from a broth culture. Inhibition zones formed on the24th hour were measured in mm's and each experimental groupwas photographed. Data were compared by Mann Whitney-U test. Results: Only water-based propolis of the present study (Group1) formed no inhibition zone against both oral pathogens andalcohol-based groups 4 and 5 formed no zone only against thelatter. Group 5 showed significantly the highest antimicrobial effectagainst S. mutans, however that group showed no influence on E.faecalis. Group 6 was found significantly more effective on E.faecalis than other propolis drops (p

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