Mehmet SAĞLAM, Doğan DOLANMAZ, Emrah KOÇAK, Burcu POYRAZ, Özgür İNAN, Niyazi DÜNDAR, Sema HAKKI

Dental implantların anatomik lokasyonlarının (mandibula ile maxilla) osseointegrasyon süresince peri-implant oluğu sıvısındaki BMP-2, BMP-7, sRANKL ve OPG seviyeleri üzerine herhangi bir etkisi yoktur. Pilot bir çalışma

Amaç: Bu çalışmanın amacı, hem maksilla hemde mandibulaya yerleştirilen implantların periimplant oluğu sıvısında osseointegrasyon süresi boyunca kemik morfogenetik protein-2 (BMP-2), BMP-7, çözünür reseptör aktivatör nükleer faktör kappa B ligandı (sRANKL) ve osteoprotegrin (OPG) seviyelerinin araştırılmasıdır.Gereç ve Yöntemler:Bu çalışmada 33 hasta (17 bayan ve 16 erkek; ortalama yaş 47.03±11.23) yer almıştır. Hem maxillaya (Grup 1/n=18) hemde mandibulaya (Grup 2/n=15) olmak üzere toplam 33 implant yerleştirilmiştir. Peri-implant oluğu sıvısı (PIS) örnekleri, modifiye plak indeksi (MPI), gingival indeks (GI) ve sondlama cep derinliği (SCD) ölçümleri cerrahiden sonra 1. ve 3. ayda alındı. BMP-2/-7, sRANKL ve OPG PIS seviyeleri ELIZA ile incelendi.Bulgular:İyileşme süresince herhangi bir komplikasyon gözlenmedi.Gruplar arasında sRANKL, OPG, BMP-2 ve BMP-7 PIS seviyeleri ve incelenen klinik parametreler açısından herhangi bir zaman periyodunda anlamlı fark gözlenmedi (p>0.05). PIS hacmi 1. ayda grup 2 de grup 1’e göre fazla iken, 3. ayda PIS hacmi grup 1 de grup 2’ye göre fazlaydı (p<0.05). PIS hacmi ile sRANKL arasında pozitif (p<0.05) ve BMP-2 ile BMP-7 arasında güçlü pozitif korelasyon (p<0.01) mevcuttu. Sonuçlar:Bu pilot çalışmanın sonuçları, dental implantların anatomik lokasyonları açısından BMP-2, BMP-7, sRANKL, ve OPG PIS seviyelerinde anlamlı bir fark gösterememiştir. Kemik ile ilişkili biyobelirteçler ve dental implantların anatomic lokasyonu arasındaki ilişkinin değerlendirilmesi için iyi dizayn edilmiş çalışmalar yapılmalıdır.

Anahtar Kelimeler:

Kemik morfogenetik proteinleri, dental implant, osteoprotegrin, reseptör aktivatör nükleer kappa B ligand

Effect of anatomical location (mandible vs maxilla) of dental implants on the BMP-2, BMP-7, sRANKL and OPG levels in periimplant crevicular fluid during osseointegration. A pilot study

Background:The aim of this study was to investigate levels of bone morphogenetic protein-2 (BMP-2), BMP-7, soluble receptor activator of nuclear factor-kB ligand (sRANKL) and osteoprotegerin (OPG) in the peri-implant crevicular fluid (PICF) of implants placed in both maxilla and mandible during the osseointegration period.Methods:Thirty-three patients (17 females and 16 males; mean age 47.03±11.23 years) were included in this study. A total of 33 implants were placed in both of maxilla (Group 1/n=18) and mandible (group 2/n=15). Peri-implant crevicular fluid (PICF) samples, modified plaque index (MPI), gingival index (GI) and probing depth (PD) measurements were obtained at 1 and 3 months after surgery. PICF levels of BMP-2/-7, sRANKL and OPG were analyzed by ELISA.Results:No complications were observed during the healing period. No significant differences were observed in the PICF levels of sRANKL, OPG, BMP-2 and BMP-7 and evaluated clinical parameters between groups at any time point (p>0.05). While PICF volume of group 2 was greater than group 1 at first month, PICF volume of group 1 was greater than group 2 at 3 months (p<0.05). There was a positive correlation between sRANKL levels and PICF volume (p<0.05) and a strong correlation between BMP-2 and BMP-7 (p<0.01).Conclusions:The results of this pilot study didn’t show any significant difference in PICF levels of BMP-2, BMP-7, sRANKL, and OPG in terms of anatomic location of dental implants. Further well-designed studies should be carried out to evaluate the relationship between bone related biomarkers and anatomic location of dental implants.

Keywords:

Bone morphogenetic proteins, dental implant, osteoprotegerin, receptor activator of nuclear factor-kappa B,

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