Kübra KISMET AKA, Çiğdem AYDIN ACAR, Meltem ÖZGÖÇMEN, Rahime ASLANKOÇ, Şükriye YEŞİLOT

RESVERATROL SIÇANLARDA OKSİDATİF STRESİ VE APOPTOZU BASKILAYARAK METOTREKSAT KAYNAKLI YUMURTALIK HASARINI HAFİFLETİR

Amaç Dişi sıçanlarda Metotreksat (MTX) kaynaklı yumurtalık hasarına karşı Resveratrolün (RES) antioksidan ve antiapoptotik etkilerinin değerlendirilmesi amaçlanmaktadır. Gereç ve Yöntem Çalışmada ratlar 7 gruba ayrıldı: Kontrol; MTX (15mg/ kg-1. gün); MTX (1. ve 3. gün); MTX (1., 3. ve 5. gün); MTX (1. gün) + RES (20mg/kg); MTX (1. ve 3. gün) + RES; MTX (1., 3. ve 5. gün) + RES. Grup 4 ve 6'da sadece bir hayvan kaldığı ve grup 7'de hiç hayvan olmadığı için bu gruplar değerlendirme dışı bırakıldı. Yumurtalık hasarı, sıçanların yumurtalık dokularında hematoksilen-eozin (H-E) ve TUNEL boyaması yapılarak değerlendirildi. Yumurtalık dokularındaki toplam oksidan/antioksidan (TOS/TAS) durumu spektrofotometrik olarak değerlendirildi. Ayrıca kandaki DNA hasarı comet testi ile değerlendirildi. Bulgular Biyokimyasal analizler sonucunda MTX gruplarında, grup 2 ve 3'te kontrol grubuna göre TOS düzeyleri artarken, grup 5'te anlamlı olarak azaldı. Öte yandan, grup 5'te grup 2 ve 3'e göre TAS düzeyi anlamlı olarak arttı. H-E boyama sonuçları, MTX kaynaklı hasarın RES (grup 5) tedavisi ile düzeldiğini gösterirken; maksimum TUNEL pozitif boyama görüntüsü; 2. ve 3. gruplarda eşit miktarlarda, ardından 5. grupta gözlemlenmiştir. Ayrıca DNA hasarını gösteren comet skoru 2. ve 3. gruplarda anlamlı olarak artarken, 5. grupta önemli ölçüde azalmıştır. Sonuç Bu çalışmanın sonuçları doğrultusunda, Resveratrol’ün antioksidan özelliğinden dolayı Metotreksat’a bağlı yumurtalık hasarına karşı koruyucu etki sağlayabileceği görülmüştür.

Anahtar Kelimeler:

Metotreksat, Resveratrol, yumurtalık hasarı, antioksidan, rat

RESVERATROL ALLEVIATES METHOTREXATE-INDUCED OVARIAN INJURY VIA SUPPRESSING OXIDATIVE STRESS AND APOPTOSIS IN RATS

Objective The study aimed to evaluate the antioxidant and antiapoptotic effects of Resveratrol (RES) against Methotrexate (MTX)-induced ovarian damage in female rats and were divided into 7 groups: Material and Method Control; MTX (15mg/kg-1st day); MTX (1st and 3rd day); MTX (1st, 3rd and 5th day); MTX (1st day) + RES (20mg/kg); MTX (1st and 3rd day) + RES; MTX (1st, 3rd and 5th day) + RES. Since there was only one animal left in groups 4 and 6 and no animals in group 7, these groups were excluded from the evaluation. Ovarian damage was evaluated by performing hematoxylin-eosin (H-E) and TUNEL staining on ovarian tissues of rats. Total oxidant/ antioxidant status in ovarian tissues was evaluated spectrophotometrically. In addition, DNA damage in blood was assessed by comet assay. Results As a result of biochemical analyzes, TOS levels increased in MTX groups, group 2 and 3, compared to the control group, while TOS level decreased significantly in group 5. On the other hand, TAS level increased significantly in group 5 compared to groups 2 and 3. H-E staining results showed that MTX-induced damage improved with RES (group 5) treatment. Maximum TUNEL positive staining image; it was observed in equal amounts in groups 2 and 3, followed by group 5. In addition, the comet score, which indicates DNA damage, increased significantly in groups 2 and 3, while it decreased significantly in group 5. Conclusion In line with the results of this study, it was observed that Resveratrol may provide protective effects against Methotrexate-induced ovarian damage due to its antioxidant properties.

Keywords:

Methotrexate, Resveratrol, ovarian damage, rat, antioxidant,

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