Türkiye’de Bulunan Bazı Sıcak Su Kaynaklarından İzole Edilen Bakterilerin Ksilanaz ve Glukoz İzomeraz Üretimi Yönünden Taranması

Bu çalışmanın amacı Türkiye’nin Ege Bölgesinde bulunan bazı sıcak su kaynaklarından elde edilen termofilik bakterilerin ksilanaz ve glukoz izomeraz üretmi yönünden taranmasıdır. Toplam 68 izolat (Anoxybacillus, Brevibacillus, Geobacillus, Aneurinibacillus, Thermus, Paenibacillus ve Proteobacter) daha önceden bu alanlardan temin edilmiş ve 16S rDNA gen dizilerine göre tanımlanmıştır. Öncelikle petri deneyi ile taranan izolatlar, ilgili enzimlere uygun besiyeri içerisinde ksilanaz için iki gün, glukoz izomeraz için ise 5-6 gün süreyle 50-60 °C’de inkübasyona tabii tutuldu ve pozitif izolatlar belirlendi. Enzim ürettiği belirlenen türler doğrulama için spektrofotometrik analizlere tabi tutuldu, birchwood ksilan ve glukoz substrat olarak kullanıldı. Türlerin çoğunluğu (59 tanesi) ksilanaz aktivitesi yönünden pozitif iken 16 türün de glukoz izomeraz aktivitesine sahip olduğu gözlendi. Izolatlardan 14 tanesinin her iki enzim yönünden de aktif olduğu belirlendi. Paenibacillus, Aneurinibacillus ve Proteobacter türlerinden hiç bir tanesi glukoz izomeraz aktivitesi göstermezken Geobacillus türlerinin glukoz izomeraz aktivitesinin oldukça yüksek olduğu belirlendi. Her iki enzim de aktivitelerini, biyoteknolojik uygulamalar için tercih edilen sıcaklık aralığı olan 50-60 °C’de gösterdi.

Anahtar Kelimeler:

Birchwood xylan, Glukoz izomeraz, Termofilik bakteri

Screening of Xylanase and Glucose Isomerase Producing Bacteria Isolated from Hot Springs in Turkey

The aim of this study was screening of xylanase and glucose isomerase producing thermophilic bacteria isolated from some hot springs located to Aegean Region of Turkey. Total sixty eight thermophilic isolates (Anoxybacillus, Brevibacillus, Geobacillus, Aneurinibacillus, Thermus, Paenibacillus and Proteobacter) were collected previously from these fields and identified based on 16S rDNA gene sequences. The isolates were screened with plate assay for determining the xylanase and glucose isomerase production abilities seperately in order to find new strains for industrial processes. After an incubation period of two days for xylanase and 5-6 days for glucose isomerase at 50-60 °C, positive strains were determined. Enzyme producing strains were confirmed by spectrophotemetric measurements with crude enzyme extracts, birchwood xylan and glucose were used as substrates. Most of the strains (59 strains) were positive for xylan degradation while only sixteen of the strains had showed glucose isomerase activity. Fourteen of the strains have showed both xylanase and glucose isomerase activity. None of the Paenibacillus, Aneurinibacillus, and Proteobacter strains were glucose isomerase positive, although the glucose isomerase activity of Geobacillus strains were notably high. Both xylanase and glucose isomerase activities have observed at 50-60 °C which is suitable for biotechnological applications.

Keywords:

Birchwood xylan, Glucose isomerase, Thermophilic bacteria,

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