Gonca Alak, Muhammed Atamanalp, Esat Mahmut Kocaman, Arzu Uçar

KARBOKSİNİN GÖKKUŞAĞI ALABALIKLARINDA (Oncorhynchus mykiss) GULUTATYON REDÜKTAZ ENZİM AKTİVİTESİ ÜZERİNE ETKİSİ

Carboxin is one of the most widely used fungicides in agriculture, but information about toxicity on fish is limited.This study assessed the effects of exposure to to carboxin on the antioxidant defence system of rainbow trout (Oncorhynchus mykiss). The fish were exposed to carboxin (3,85 ppm) for seven days. And antioxidant parameter (glutathione reductase, (GR)) was measured in hepar. The results indicated that carboxin exposure significantly affected the activity of GR in fish hepar (p< 0.01). Thus, it was assumed that carboxin caused oxidative stress in rainbow trout and GR enzyme played a role in protection against carboxin toxicity.

Anahtar Kelimeler:

Karboksin, gökkuşağı alabalığı, toksisite, antioksidan enzim, GR

EFFECTS OF CARBOXIN ON GLUTATHIONE REDUCTASE ENZYME ACTIVITY IN RAINBOW TROUT (Oncorhynchus mykiss)

Carboxin is one of the most widely used fungicides in agriculture, but information about toxicity on fish is limited. This study assessed the effects of exposure to to carboxin on the antioxidant defence system of rainbow trout (Oncorhynchus mykiss). The fish were exposed to carboxin (3,85 ppm) for seven days. And antioxidant parameter (glutathione reductase, (GR)) was measured in hepar. The results indicated that carboxin exposure significantly affected the activity of GR in fish hepar (p< 0.01). Thus, it was assumed that carboxin caused oxidative stress in rainbow trout and GR enzyme played a role in protection against carboxin toxicity.

Keywords:

Carboxin, rainbow trout, toxicity, antioxidant enzyme, GR,

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EPA. Environmental Protection Agency and Toxic Substances September 2004.
CEYHUN, S.B., ŞENTÜRK, M., EKİNCİ, D., ERDOĞAN, O., ÇİLTAŞ, A., KOCAMAN, E.M., Deltamethrin attenuates antioxidant defense system and induces the expression of heat shock protein 70 in rainbow trout, Comp. Biochem. Physiol., 152, 215, 2010.
TALAS, Z.S., ORUN, I., OZDEMİR, İ., ERDOĞAN, K., ALKAN, A., YILMAZ, I., Antioxidative role of selenium against the toxic effect of heavy metals (Cd+2, Cr+3) on liver of rainbow trout (Oncorhynchus mykiss Walbaum 1792), Fish. Physiol. Biochem., 34, 217, 2008.
DOYOTTE, A., COSSU, C., JACQUIN, M.C., BABUTB, M., VASEURAL, P., Antioxidant enzymes, glutathione and lipid peroxidation as relevant biomarkers of experimental or field exposure in the gills and the digestive gland of the freshwater bivalve unio tumidus, Aquat. Toxicol., 39, 93, 1997. ZWART, L.L.D., MEERMAN, J.H.N., COMMANDEUR, J.N.M., VERMEULEN, N.P.E., Biomarkers of free radical damage applications in experimental animals and in humans, Free Radıcal. Bıo. Med., 27, 202, 1999.
ORUÇ, E.Ö., SEVGİLER, Y., ÜNER, N., Tissuespecific oxidative stress responses in fish exposed to 2,4D and azinphosmethyl, Comp. Biochem. Phys., 137, 43, 200 ÇINKILOĞLU, E. Cyprinus carpio’da beyin dokusunda fenthionun antioksidant savunma sistemi, lipid peroksidasyonu ve asetilkolinesteraz enzim aktivitesine n-asetilsistein modülatörlüğünde etkileri. Çukurova Üniversitesi, Fen Bilimleri Enstitüsü. 2007.
WIEGAND, C., KRAUSE, E., STEINBERG C., PFLUGMACHER, S., Toxicokinetics of atrazine in embryos of the zebrafish (Danio rerio), Ecotoxicol. Environ. Safe.. 49, 199, 2001.
RAO,J.V.,Sublethaleffectsofanorganophosphorusinse cticide (RPR- I)onbiochemical parametersoftilapia, Oreochromis mossambicus, Comp.Biochem. Physiol., 143,492, 2006.
SETH, V., BANERJEE, B.D., CHAKRAVORTY, A.K., Lipid peroxidation, free radical scavenging enzymes, and glutathione redox system in blood rats exposed to propoxur, Pestıc. Bıochem. Phys., 71, 133, 200 JOS, A., PICHARDO, S., PRIETO, A.I., REPETTO, G., VAZQUEZ, C.M., MORENO, I., CAMEAN, A.M., Toxic cyanobacterial cells containing microcystins induce oxidative stress in exposed tilapia fish (Oreochromis sp.) under laboratory conditions, Aquat. Toxicol., 72, 261, 2005.
PEIXOTO, F., ALVES-FERNANDES, D., SANTOS, D., FONTAÍNHASFERNANDES, A., Toxicological Effects of Oxyfluorfen on Oxidative Stress Enzymes in Tilapia Oreochromis niloticus. Pestıc. Bıochem. Phys., 85, 91, 2006.
LI, Z., ZLABEKA, V., GRABICA, R., LIA, P., MACHOVAA, J., VELISEKA, J., RANDAK, T., Effects of exposure to sublethal propiconazole on the antioxidant defense system and Na+– K+-ATPase activity in brain of rainbow trout, Oncorhynchus mykiss, Aquat. Toxicol.,98, 297,2010.
FIGUEIREDO-FERNANDES,A.,FONTAI´NHASFERNANDES, A., PEIXOTO, F., ROCHA, E., REISHENRIQUES, M.A., Effects of gender and temperature on oxidative stress enzymes in Nile tilapia (Oreochromis niloticus) exposed to paraquat, Pestıc. Bıochem. Phys., 85, 97, 2006.
LOPES, P.A., PINHEIRO, T., SANTOS, M.C., MATHIAS, M.D.L., COLLARESPEREIRA, M.J., VIEGAS-CRESPO, A.M., Response of antioxidant enzymes in freshwater fish populations (Leuciscus alburnoides) to inorganic pollutants exposure, Sci. Total Environ., 280, 153, 2001.