Çeşitli ligandları kullanarak Fe3+, Al3+ ve Cu2+ nin bir arada spektrofotometrik tayinleri için yöntem geliştirilmesi ve elde edilen verilerin en küçük kareler kalibrasyon yöntemi (PLS) ve temel bileşen regresyon (PCR) yöntemi ile değerlendirilmesi

Bu çalışmada iki farklı ligand alizarin red (AR) ve eriochrome black T (ECR) denenmiş, bunlardan ECR ile dahadoğru sonuçlar elde edildiğinden çalışmalara ECR ile devam edilmiştir. Fe3+, Al3+ ve Cu2+ ' nın ECR ile komplekslerioluşturularak spektrofotometrik yöntemle bir arada tayinleri yapılmıştır. Uygun parametreler belirlenmiş (ligandmiktarı, pH, bekleme süreleri, metal derişimi ile absorbans arasındaki ilişki ve yabancı iyon etkisi) ve optimum koşullarsağlanmıştır. Optimum şartlar sağlandıktan sonra Fe3+, Al3+ ve Cu2+ içeren karışımların spektrofotometrik tayinlerigerçekleştirilmiş ve veriler en küçük kareler kalibrasyon yöntemi (PLS) ve temel bileşim regresyon (PCR) yöntemlerikullanılarak değerlendirilmiştir. Uygulanan kemometrik yöntemler hızlı, basit ve çıkan sonuçlar uygulanabilirdir.

Anahtar Kelimeler:

Demir, alüminyum, bakır, spektrofotometri, kemometrik yöntem

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In this study, two different ligand alizarin red (AR), and eriochrome black T (ECR) tried these ECR more accurate results are obtained from studies ECR continued with. Fe3+ , Al 3+ and Cu2+ complexes formed by the ECR were determined by a combination of spectrophotometric method. Accordance with determined parameters (ligand concentration, pH, waiting times, the relationship between absorbance and concentration of metal ion effect and foreign) are provided and the optimum conditions. After establishing the optimum conditions Fe3+ , Al 3+ and Cu2+ containing mixtures spectrophotometric determinations and the data calibration method of least squares (PLS) regression, and principal component regression (PCR) methods were used. Chemometric methods are applied in a fast, simple, and the results are applicable.

Keywords:

Iron, aluminum, copper, spectrophotometry, chemometric method.,

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(REFERENCES) Zarei K., Atabati M., Malekshabani Z., (2006) "Simultaneous spectrophotometric determination of iron, nickel and cobalt in micellar media by using direct orthogonal signal correction-partial least squares method", Anal. Chim. Acta, 556, 247-2
Scancar J., Benedik R.M.M., Krızaj I., (2003) "Total metal concentrations in serum of dialysis patients anf fractionation of Cu, Rb, Al, Fe and Zn in spent continuous ambulatory peritoneal dialysis fluid", Talanta, 59, 355-364.
Tahan J.E., Granadilla V.A., Romero R.A., (1994) "Electrothermal atomic absorption spectrometric determination of Al, Cu, Fe, Pb, V and Zn in clinical samples and certified environmental reference materials", Anal. Chim. Acta, 295, 187-197.
Nagaoku M.H., Maitani T., (2000) "Binding patterns of co-existing aluminium and iron to human serum transferrin studied by HPLC-high resolution ICP-MS", Analyst, 125,1962-1965.
Coscaine A.R, Andrade J.C., Poppi R.J.,(2002)
"PLS regression using real sample calibration for aluminium and iron determination in plant extracts", Analyst, 127,135-139. Kendüzler E., Türker A.R., (2003) "Atomic absorption spectrophotometric determination of trace copper in waters, aluminium foil and tea samples after preconcentration with 1-nitroso-2napthol-3,6-disulfonic acid on Amsersorb 572", Anal. Chim. Acta, 480, 259-266.
Ryan E., Meaney M., (1992) "Determination of trace levels of copper (II), aluminium (III) and iron (III) by reversed phase high performance liquid chromatography using a novel on-line sample preconcentration technique", Analyst, 117, 1435-1439.
Bond A.M., Nagaosa Y., (1985) "Determination of aluminium, copper and manganase in biological and other samples as 8-quinolinol complexes by high-performance liquid chromatography with electrochemical and spectrohotometric detection", Anal. Chim. Acta, 178, 197-208.
Coscine A.R., Andrade J.C., Poppi R.J., Mello C., Raji B.V., Mbreu M.F., (2000) " Multivariate calibration applied to a highly interfering chemical system: The simultaneous spectrophotometric determination of aluminium and iron in plants using xylenol orange and partial least squares regression", Anal. Chim. Acta, 423, 31-40.
Wang N., Liang W., (1993) "Simultaneous thirdderivative spectrophotometric determination of copper and nickel in iron alloys and aluminium alloy", Talanta, 40(6), 897-899.
Thakur M., Deb M.K., (1999) " The use of 1jpyridyl-(2)-azoj-naphthol-(2) in the presence of
Tx-100 and N-N'-diphenylbenzamidine for the specrophotometric determination of copper in real samples", Talanta, 49, 561-569. Limson J., Nyokong T., Daya S.,(1998) "The interaction of melatonin and its precusors with aluminium, cadmium, copper, iron, lead, and zinc: An adsorptive voltametric study", Journal of Pineal Research, 24(1), 15-21.
Safavi A., Abdollahi H., Mirzajani R., (2006) "Simultaneous spectrophotometric determination of Fe(III), Al (III) and Cu(III) by partial least squares calibration method", Spectrochimica Acta Part A, 63, 196-199.
Reddy V.K., Reddy S.M., Reddy P.R., Reddy T.S., (2000) "Simultaneous spectrophotometric determination of copper (II), vanadium (V) and iron (III) using 2-hydroxy-1-naphthaldehyde benzoylhydrazone", Journal of Analytical Chemistry, 55(5), 435-439.
Honorato R.S., Carneiro J.M.T., Zagatto E.A.G., (2001) "Spectrophotometric flowbatch determination of aluminium in plant tissues exploiting a feedback mechanism", Anal. Chim. Acta, 441, 309–315.
Rodrigues J.L., de Magalhaes S.C., Luccas P.O.,(2005) "Flow injection spectrophotometric determination of Al in hemodialysis solutions", Journal of Pharmaceutical and Biomedical Analysis, 36, 1119–1123.