Ebubekir DİRİCAN, Burak KANKAYA, Zeynep TATAR

Meme Kanseri Hastalarında hTERT Gen Ekspresyonunun Klinikopatolojik Önemi

Amaç: Bu çalışma, meme kanserinde insan telomeraz ters transkriptaz (hTERT) genin mRNA ekspresyonunun klinik önemini incelemeyi ve klinikopatolojik rolünü araştırmayı amaçlamıştır. hTERT geni hücre proliferasyonu, apoptoz ve hücre invazyonu gibi olaylarda rol almaktadır. Yüksek hTERT mRNA ekspresyonunun meme kanseri, mide, akciğer, glioblastoma, baş ve boyun gibi solid tümörlerde kötü prognoz ile ilişkili olduğu bildirilmektedir. Gereç ve Yöntemler: 59 meme kanser ve sağlıklı kan örneklerinin RNA izolasyonları gerçekleştirildi. hTERT geni mRNA ekspresyonu Gerçek Zamanlı Polimeraz Zincir Reaksiyonu (RT-PZR) yöntemi ile analiz edildi. mRNA ekspresyon sonuçları 2-∆∆ct metoduyla kanserli ve sağlıklı örneklerde değerlendirildi. Klinik veriler toplandı ve bu verilerin hTERT gen ekspresyonu ile ilişkisi istatiksel olarak değerlendirildi. Bulgular: Meme kanser hastalarında hTERT geni mRNA ekspresyonu sağlıklı örneklere kıyasla anlamlı derecede yüksekti (p=0,0211). Düşük hTERT geni ekspresyonu östrojen reseptör (ÖR) (+) ve insan epidermal büyüme faktör reseptörü 2 (HER 2) (+) olan hastalarda anlamlı derecede ilişkiliydi (sırasıyla p=0,0464; p=0,0080). hTERT geni ekspresyonu histolojik derece (grade) 3 hastalarda derece 2’ye kıyasla yüksek anlamlı bulundu (p=0,0389). Bununla birlikte hTERT ekspresyonun bazal benzeri histolojik tipte luminal A ve luminal B’ye kıyasla anlamlı derecede yüksek olduğu gösterildi (sırasıyla p=0,0160; p=0,0008). Sonuç: Çalışmamızın bulguları, meme kanser hastalarında hTERT gen ekspresyonunun sağlıklı örneklere göre anlamlı olarak daha yüksek olduğunu gösterdi. Anormal hTERT gen ekspresyonu kanserojenez veya teşhiste önemli bir rol oynayabilir.

Anahtar Kelimeler:

Meme kanseri, hTERT, ÖR, PZR, bazal benzeri, luminal

Clinicopathological Significance of hTERT gene expression in Breast Cancer Patients

Aim: This study aimed to examine the clinical significance of the mRNA expression of the human telomerase reverse transcriptase (hTERT) gene in breast cancer (BCa) and to investigate its clinicopathological role. The hTERT gene is involved in events such as cell proliferation, apoptosis and cell invasion. It has been reported that high hTERT mRNA expression is associated with poor prognosis in solid tumors such as BCa, stomach, lung, glioblastoma, head and neck. Material and Methods: RNA isolations of 59 BCa and healthy blood samples were performed. hTERT gene mRNA expression was analyzed by Real-Time-Polymerase Chain Reaction (RT-PCR) method. mRNA expression results were evaluated in cancer and healthy samples by the 2-∆∆ct method. Clinical data were collected and the relationship of these data with hTERT gene expression was evaluated statistically. Results: hTERT gene mRNA expression in BCa patients was significantly higher than in healty samples (p=0,0211). Low hTERT gene expression was significantly associated with estrogen receptor (ER) (+) and human epidermal growth factor receptor 2 (HER 2) (+) patients (p=0.0464; p=0.0080, respectively). hTERT gene expression was found to be higher in histological grade 3 patients compared to grade 2 (p=0.0389). Moreover, hTERT expression was shown to be significantly higher in basal-like histological type compared to luminal A and luminal B (p=0.0160; p=0.0008, respectively). Conclusion: The findings of our study showed that hTERT gene expression was significantly higher in BCa patients than in healthy samples. Abnormal hTERT gene expression may play an important role in carcinogenesis or diagnosis.

Keywords:

Breast cancer, hTERT, PCR, basal like, luminal,

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