The effectiveness of synchronızation of estrus vıa prostaglandin F2α administration and timed artificial inseminaton in multıpar and nullipar Akkaraman breed ewes
Özet : Sunulan çalışmada; prostaglandin F2α (PGF2α) ile östrus senkronizasyonu ve sabit zamanlı tohumlamanın multipar ve nullipar Akkaraman ırkı koyunlarda, etkinliğinin araştırılması amaçlandı. Araştırmada 10 adet multipar (2-4 yaşında, Grup 1) ve 10 adet nullipar koyun (1,5-2 yaşında, Grup 2) olmak üzere iki grupta toplam 20 adet hayvan kullanıldı. Tüm hayvanlara 9 gün arayla 125 μg Cloprostenol (Estrumate® DİF İstanbul Türkiye) enjekte edildi. Sperma altı adet koçtan (2-3 yaşında) suni vagen yardımı ile alındı ve 150 milyon/0,5 ml dozda sulandırıldı. Koyunlar ikinci PGF2α uygulamasından 42 saat sonra intravaginal yolla tohumlandı. Kandaki progesteron düzeyleri hormon enjeksiyonları anında ve 3’er gün sonrasında değerlendirildi. Gebelikler, tohumlamadan 35 gün sonra, ultrasonografi ile teşhis edildi. Gebelik oranları multipar koyunlarda % 40, nulliparlarda % 20 olarak belirlendi (p>0.05). Birinci PGF2α uygulaması öncesinde, birinci PGF2α uygulamasından üç gün sonra, ikinci PGF2α uygulamasından önce ve ikinci PGF2α uygulamasından üç gün sonra multipar-nullipar koyunlarda ortalama progesteron düzeyleri sırasıyla, 2.67-3.67 ng/ml (p>0.05), 0.96-0.86 ng/ml (p>0.05), 6.26-5.24 ng/ml (p
Multipar ve nullipar Akkaraman ırkı koyunlarda prostaglandin F2α ile östrus senkronizasyonu ve sabit zamanlı tohumlamanın etkinliği
Summary : In present study it was aimed to investigate the effectiveness of synchronization of estrus via prostaglandin F2α(PGF2α) administration and timed artificial insemination (TAI) in multipar and nullipar Akkaraman breed sheep. The study used a total of 20 animals which were divided into two groups: Group 1=10 multipar ewes (2-4 years old) and Group 2=10 nullipar ewes (1.5-2 years old). All animals were injected with 125μg Cloprostenol (Estrumate® DIF İstanbul, TURKEY) at nine day intervals. Ewes were inseminated vaginally 42 hours after the second PGF2α administration with the semen which were collected from six purebred Akkaraman rams (2-3 years old) and diluted at 150x 106 motile spermatozoa/0.5 ml dose. Progesterone levels in blood samples were assessed at the moment of hormone injections and after three days. Thirty five days after the inseminations, pregnancies were diagnosed via ultrasonography. Pregnancy rates were found to be 40 % for multipar and 20 % for nullipar ewes (p>0.05). Progesterone levels in multipar and nullipar animals at the first PGF2α administration, three days after the first PGF2α administration, at the second PGF2α administration, and three days after the second PGF2α administration were measured as (median) 2.67-3.67 ng/ml (p>0.05), 0.96-0.86 ng/ml(p>0.05), 6.26-5.24 ng/ml (p
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- 1. Colakoglu N, Ozbeyaz C. Akkaraman ve Malya koyunlarının bazı verim özelliklerinin karşılaştırılması J Vet Anim Sci 1999, 23:351-360.
- 2. Esen F, Bozkurt T. Akkaraman ırkı koyunlarda flushing ve östrus senkronizasyonu uygulamasının dölverimi üzerine etkisi. Turk J Vet Anim Sci 2001, 25:365-368.
- 3. Leethongdee S, Khalid M, Bhatti A, et al. The effects of the prostaglandin E analogue Misoprostol and follicle-stimulating hormone on cervical penetrability in ewes during the peri -ovulatory period. Theriogenology 2006, 67: 767-777.
- 4. Menchaca A, Miller V, Gil J, et al. Prostaglandin F2α treatment associated with timed artificial insemination in ewes. Reprod Dom Anim 2004, 39:352-355.
- 5. Deligiannis C, Valasi I, Rekkas CA, et al. Synchronization of ovulation and fixed time intrauterine insemination in ewes. Reprod Dom Anim 2005, 40:6-10.
- 6. Thompson JM, Meyer H. Body condition scoring of sheep. http://oregonstate.edu/dept/ animal-sciences/bes.htm 2007.
- 7. Paulenz H, Söderquist L, Pérez-Pé R, et al. Effect of different extenders and storage temperatures on sperm viability of liquid ram semen. Theriogenology 2002, 57:823-836.
- 8. Gonzalez-Bulnes A, Veiga-Lopez A, Garcia P, et al. Effects of progestagens and prostaglandin analogues on ovarian function and embryo viability in sheep. Theriogenology 2005, 63:2523-2534.
- 9. Greyling JPC, Erasmus JA, Taylor GJ, et al. Synchronization of estrus in sheep using progestagen and inseminating with chilled semen during the breeding season. Small Rumin Res 1997, 26(1-2):137-143.
- 10. Luther JS, Grazul-Bilska AT, Kirsch RM, et al. The effect of GnRH, eCG and progestin type on estrus synchronization following laparoscopic AI in ewes. Small Rum Res 2007, 72(2-3): 227-231.
- 11. Naqvi SMK, Joshi A, Bag SR, et al. Cervical penetration and transcervical AI of tropical sheep (Malpura) at natural oestrus using frozen-thawed semen. Small Rumin Res 1998, 29: 329-333.
- 12. Anel L, Alvarez M, Martinez-Pastor F, et al. Improvement Strategies in Ovine Artificial Insemination. Reprod Dom Anim 2006, 41:30-42.
- 13. Anel L, Kaabi M, Abroug B, et al. Factors influencing the success of vaginal and laparoscopic artificial insemination in churra ewes: a field assay. Theriogenology 2005, 63: 1235-1247.
- 14. Fair S, Hanrahan JP, O’Meara CM, et al. Differences between Belclare and Suffolk ewes in fertilization rate, embryo quality and accessory sperm number after cervical or laparoscopic artificial insemination. Theriogenology 2005, 63:1995-2005.
- 15. Barrett DMW, Bartlewski PM, Cook SJ, et al. Ultrasound and endocrine evaluation of the ovarian response to PGF2α given at different stages of the luteal phase in ewes. Theriogenology 2002, 58:1409-1424.
- 16. Paulenz H, Söderquist L, Ådnøy T, et al. Effect of milk- and TRIS-based extenders on the fertility of sheep inseminated vaginally once or twice with liquid semen. Theriogenology 2003, 60:759-766.
- 17. Paulenz H, Söderquist L, Ådnøy T, et al. Effect of vaginal and cervical deposition of semen on the fertility of sheep inseminated with frozenthawed semen. Vet Rec 2005, 156: 372-375.
- 18. Yıldız S, Saatci M, Uzun M, et al. Effects of ram introduction after the second prostaglandin F2α injection on day 11 on the LH surge characterisrics in fat-tailed ewes. Reprod Dom Anim 2003, 38:54-57.