Ayhan ATASEVER, Gökhan SEYFİ, Görkem EKEBAŞ

TAVŞANLARDA FARKLI EIMERIA STIEDAI SUŞLARI İLE OLUŞTURULAN DENEYSEL KARACİĞER KOKSİDİYOZUNDA PATOLOJİK VE BİYOKİMYASAL BULGULARIN KARŞILAŞTIRILMASI

Çalışma, tavşanlarda E. stiedae’nin saha ve standart suşuyla oluşturulan karaciğer koksidiyozisinde klinik, biyokimyasal ve patolojik bulguları karşılaştırmak amacıyla yapılmıştır. Çalışmada, 1-1.5 kg, 6-8 haftalık 36 adet yeni Zelanda tavşanı kullanılmıştır. Hayvanlar 3 gruba ayrılmıştır. Birinci grup kontrol tutulurken, sırasıyla ikinci ve üçüncü gruptaki tavşanlara, 1 ml’sinde 50.000 sporlanmış oosit içeren saha ve standart suştan, 1 ml direkt sonda ile ağızdan mideye verilmiştir. Makroskopik ve histolojik olarak Eimeria stiedae standart suşu inokule edilen üçüncü gruba göre, saha suşu inokule edilen ikinci grupta daha şiddetli karaciğer lezyonları belirlenmiştir. Etken inokulasyonunun 20. ve 30. günlerinde; serum gamma glutamiltransferaz (GGT), alanin aminotransferaz (ALT) ve aspartat aminotransferaz (AST) enzim aktivitelerinde grup bir ile grup ikideki tavşanlar arasında istatistiksel önemli bir fark bulunmamıştır. Buna karşın, etken inokulasyonunun 20. ve 30. günlerinde, grup birdeki tavşanlara ait serum GGT, ALT ve AST enzim aktivitelerine göre grup üçteki tavşanların enzim aktivitelerinde önemli bir artış (p<0.001) belirlenmiştir. Tavşanlarda E. stiedae’nin saha ve standart suşu ile oluşturulan karaciğer koksidiyozisinde bazı klinik, biyokimyasal ve patolojik değişikliklerin, oluşan enfeksiyonun şiddeti ile farklılık gösterebileceğinin ortaya konulduğu ilk çalışmadır

Anahtar Kelimeler:

Biyokimyasal parametre, Eimeria stiedae, patoloji, tavşan.

Comparison of Pathological and Biochemical Findings in Experimentally Induced the Liver Coccidiosis by Different Types of Eimeria Stiedae Strains in the Rabbits

The aim of this study was to compare clinical, biochemical and pathological findings of the liver coccidiosis induced by field and standard strain of the Eimeria stiedae in the rabbits. Thirty-six New Zealand rabbits weighing 1.5 kg and aged 6-8 weeks were used. The rabbits were allocated into three groups, the first of which was healthy control group. One ml of inoculum of field strain and standard strain containing 50 000 sporulated E.stiedae oocysts per ml were administered orally by a catheter to each rabbit in the second group and third group, respectively. Liver lesions were more severe in the second group of the Eimeria stiedae field strain inoculated than the third group in which the Eimeria stiedae standard strain was inoculated macroscopically and histologically. No statistical difference was found in serum GGT, ALT and AST enzyme activities between the first and second group at day 20 and 30 after the inoculation. However, at day 20 and 30 after the inoculation, a significant increase (p<0.05) was found in serum GGT, ALT and AST enzyme activities of the rabbits in the third group compared to those in group one. This is the first study to suggest that some clinical, biochemical and pathological changes in the liver coccidiosis induced with a field and standard strain of E. stiedae may vary depending on the severity of the infection.

Keywords:

Biochemical parameter, Eimeria stiedae, pathology, rabbit.,

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