PERİAPİKAL LEZYONLU DİŞLERDE CANDİDA ALBİCANS’İN ELEKTRONİK-BURUN TEKNOLOJİSİ VE MİKROBİYOLOJİK KÜLTÜR YÖNTEMİ İLE DEĞERLENDİRİLMESİ
Pulpa ve periradiküler doku hastalıklarının çoğu hem aerop hem de anaerop mikroorganizmaları içeren karışık bir mikroflora ile ilişkilidir. Hekim tedavi ile ilgili etkili bir yöntem belirleyebilmek için mikroorganizma varlığı ile endodontik hastalık arasındaki yakın ilişkiyi kavramalıdır. Endodontik enfeksiyonlardaki bakteri türlerini ortaya çıkarmak için kültür ve moleküler metodlar kullanılmaktadır. Son yıllarda moleküler yöntemlerdeki gelişmelere rağmen her iki metodun da bir takım dezavantajları bulunmaktadır. Elektronik burun yöntemi, insan doku duyusunu taklit edebilen bir elektronik sistemi tanımlar. Elektronik burun sistemleri tıp alanında ve mikrobiyolojik araştırmalarda başarıyla kullanılmaktadır. Bu sistemlerin en önemli özelliği, koku çeşitlerini çok kısa zamanda insan burnundaki hassasiyet derecesinde ayrıştırabilmesi ve sonucu objektif olarak sunmasıdır. Bu çalışmada insan diş köklerinden alınan örnekler elektronik burun sistemine tabi tutulmuş ve makroskobik ve mikroskobik analizleri yapılmıştır. Örneklerde mikroskopi ile tespit edilen Candida varlığının, elektronik burun tarafından da tespit edilip edilemediği araştırılmıştır. Çalışmada kullanılan elektronik burun sisteminin var olan teknolojisi ile kök kanallarında var olan mikroorganizma tipini tespit edemediği ve ileri teknik donanımlara ihtiyacı olduğu anlaşılmıştır.
Electronics-Nose of Candida Albicans in Periapical Lesioned Threads Evaluation by Technology and Microbiological Culture Method
Most diseases of pulp and periradicular tissues are associated with mixed micro flora including both aerobic and anaerobic microorganisms. Clinicians must understand the close relationship between the presence of microorganism and endodontic disease process to determine an effective procedure with respect to the treatment. Culture and molecular methods are used to detect bacterial species in root canal infections. Despite the recent advances in molecular methods, both culture and molecular methods have several disadvantages. The term electronic nose describes an electronic system that is able to mimic the human sense of smell. Electronic nose systems have already been used with success in the medical science and microbiological research. The most important feature of an electronic nose system is its ability to detect different odour types in a short period of time almost with the sensivity of human nose. Furthermore, the result obtained using electronic nose system are objective. In this study, samples collected from root canals were compared both macroscopically and microscopically and also with an electronic nose system in order to detect the presence of candida species in teeth with periapical lesions. The electronic nose device used in this study was found insufficient to detect intra canal candida species and needs more sensitive chemical sensors.
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- 1. Sjögren U, Hänström L, Happonen R, Sundqvist G.
Extensive bone loss associated with periapical infection with Bacteroides gingivalis: a case report. Int
Endod J 1990;23:254-262.
- 2. de Oliveira JCM, Siqueira JF, Alves GB, Hirata R,
Andrade AF. Detection of Porphyromonas endodontalis in infected root canals by 16S rRNA genedirected polymerase chain reaction. J Endon
2000;26:729-732.
- 3. Griffee MB, Patterson SS, Miller CH, Kafrawy AH,
Newton CW. The relationship ofBacteroides melaninogenicus to symptoms associated with pulpal necrosis. Oral Surg Oral Med Oral Pathol 1980;50:457-
461.
- 4. Sundqvist G, Johansson E, Sjögren U. Prevalence of
black-pigmented bacteroides species in root canal
infections. J Endod 1989;15:13-19.
- 5. Odell LJ, Baumgartner JC, Xia T, David LL. Survey for
collagenase gene prtC in Porphyromonas gingivalis
and Porphyromonas endodontalis isolated from
endodontic infections. J Endod 1999;25:555-558.
- 6. Waltimo T, Siren E, Torkko H, Olsen I, Haapasalo M.
Fungi in therapy‐resistant apical periodontitis. Int
Endod J 1997;30:96-101.
- 7. Chávez de Paz L. Gram‐positive organisms in endodontic infections. End Topics 2004;9:79-96.
- 8. Siqueira JF, Sen BH. Fungi in endodontic infections.
Oral Surg Oral Med Oral Path Oral Rad Endod
2004;97:632-641.
- 9. Möller A. Microbiological examination of root canals
and periapical tissues of human teeth. Methodological studies. Odont Tids 1966;74:15-19
- 10. Nair PR, Sjögren U, Krey G, Kahnberg K-E, Sundqvist
G. Intraradicular bacteria and fungi in root-filled,
asymptomatic human teeth with therapy-resistant
periapical lesions: a long-term light and electron
microscopic follow-up study. J Endod 1990;16:580-
588.
- 11. Tanaka M, Anguri H, Nonaka A, Kataoka K, Nagata H,
Kita J, et al. Clinical assessment of oral malodor by
the electronic nose system. J Dent Res 2004;83:317-
321.
- 12. Sundqvist G, Figdor D, Persson S, Sjögren U. Microbiologic analysis of teeth with failed endodontic
treatment and the outcome of conservative retreatment. Oral Surg Oral Med Oral Pathol Oral Rad
Endod 1998;85:86-93.
- 13. Baumgartner JC, Watts CM, Xia T. Occurrence of Candida albicans in infections of endodontic origin. J
Endod 2000;26:695-698.
- 14. Trihaas J, Vognsen L, Nielsen P. Electronic nose: New
tool in modelling the ripening of Danish blue cheese.
Int Dairy J 2005;15:679-691.
- 15. Stuetz R, Fenner R, Engin G. Characterisation of
wastewater using an electronic nose. Wat Res
1999;33:442-452.
- 16. Gardner JW, Shin HW, Hines EL. An electronic nose
system to diagnose illness. Sens Act Chemical
2000;70:19-24.
- 17. Pavlou A, Turner A, Magan N. Recognition of anaerobic bacterial isolates in vitro using electronic nose
technology. Letters in Applied Microbiology.
2002;35(5):366-369.
- 18. Yamada Y, Takahashi Y, Konishi K, Katsuumi I. Association of odor from infected root canal analyzed by
an electronic nose with isolated bacteria. J Endod
2007;33:1106-1109.
- 19. Aksebzeci BH, Asyalı MH, Kahraman Y, Er Ö, Kaya E,
Özbilge H, et al. Classification of root canal microorganisms using electronic-nose and discriminant
analysis. Biomed Eng Online 2010;9:77.
- 20.Baumgartner J, Hutter J, Siqueira J. Endodontic microbiology and treatment of infections. Pathways of
the Pulp 2006;2:580-607.
- 21. Byström A, Sundqvist G. Bacteriologic evaluation of
the efficacy of mechanical root canal instrumentation in endodontic therapy. Euro J Oral Sci
1981;89:321-328.
- 22. Dutta R, Hines EL, Gardner JW, Boilot P. Bacteria
classification using Cyranose 320 electronic nose.
Biomed Eng Online 2002;1:4.
- 23. Hancock H, Sigurdsson A, Trope M, Moiseiwitsch J.
Bacteria isolated after unsuccessful endodontic
treatment in a North American population. Oral Surg
Oral Med Oral Path Oral Rad Endod 2001;91:579-
586.
- 24. Cheung G, Ho M. Microbial flora of root canal–
treated teeth associated with asymptomatic periapical radiolucent lesions. Molecular Oral Microb
2001;16:332-337.
- 25. Sen B, Piskin B, Demirci T. Observation of bacteria
and fungi in infected root canals and dentinal tubules by SEM. Dent Trauma 1995;11:6-9.
- 26. Şen BH, Safavi KE, Spångberg LS. Growth patterns of
Candida albicans in relation to radicular dentin. Oral
Surg Oral Med Oral Path Oral Rad Endod 1997;84:68
-73.