Leyla PAŞAYEVA, Ayşe Kübra KARABOĞA ARSLAN

CAPPARIS OVATA DESF. VAR. PALAESTINA ZOH. BİTKİSİNİN ÇİÇEK VE MEYVE EKSTRELERİNİN ENZİM İNHİBİTÖR ÖZELLİKLERİ VE ANTİOKSİDAN ETKİLERİNİN ARAŞTIRILMASI

Bu çalışmada Capparis ovata var. palaestina bitkisinin çiçek ve meyvelerinden hazırlanan etanol ekstresinin α-amilaz ve α-glikozidaz inhibitör etkisi ve antioksidan özelliği araştırılmıştır. Ekstrelerin antioksidant etkisi DPPH●, ABTS●+ ve FRAP testleri kullanılarak değerlerndirilmiştir. Sonuçlara göre en yüksek α-amilaz ve α-glikozidaz etki meyve ekstresinde bulunmuştur (Sırasıyla, IC50=271.72±0.41 µg/ml and 142.07±0.845 µg/ml). Ayrıca en yüksek TPC (26.40±0.02mgGAE/gextract) ve TFC (8.33±0.30 mgCA /gextract) değerleri ve DPPH● (32%) etkisi de aynı ekstrede görülmüştür. Bununla birlikte çiçek ekstresi en yüksek FRAP değerleri (1 mg/ml konsantrasyonda 262.69±2.27 mmol Fe2+ eşdeğer) ve ABTS+● (0,5 mg/ml konsantrasyonda 0.18±0.01 µM Troloks’a eşdeğer) aktivite göstermiştir. Sonuç olarak, yapılan çalışmanın bu bitki üzerinde ileride yapılacak çalışmalara ışık tutacağı düşünülmektedir.

Anahtar Kelimeler:

Capparaceae, Diyabet, DPPH, α-amilaz, α-glikozidaz

INVESTIGATION OF ENZYME INHIBITORY PROPERTIES AND ANTIOXIDANT ACTIVITY OF CAPPARIS OVATA DESF. VAR. PALAESTINA ZOH. FLOWER AND FRUIT EXTRACTS

In this study, the inhibiting effect on α-amylase and α-glucosidase and the antioxidant activity of ethanol extracts from flower and fruit of Capparis ovata var. palaestina was investigated. The antioxidant activity of extracts was tested with DPPH●, ABTS●+ and FRAP tests. According to the results, the highest α-glucosidase and α-amylase activity were found in fruit extract (IC50=271.72±0.41 µg/mL and 142.07±0.85 µg/mL, respectively). Also, the highest TPC (26.40±0.02mgGAE/gextract) and TFC (8.33±0.30 mgCA /gextract) was found in the same extract and this extract showed the highest DPPH● activity (%32). Although, flower extract showed the highest FRAP values (at a concentration of 1 mg/mL equivalent to 262.69±2.27 mmol Fe2+) and ABTS+● (at a concentration of 0.5 mg/mL equivalent to 0.18±0.01 µM Trolox) activity. In conclusion, it is thought that these studies will be shed light on future studies on this species.

Keywords:

Capparaceae, Diabetes, DPPH, α-amylase, α-glucosidase,

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