Ayşe Nedret KOÇ, Aslıhan GÜLTEKİN, Mustafa Altay ATALAY

Candida türlerinin tanımlanmasında kromojenik besiyerinin değerlendirilmesi

Mayalar morfolojik, biyokimyasal ve fizyolojikbenzerliklerinden dolayı sıklıkla karıştırılabilir. Buçalışmanın amacı, sistemik kandidoz şüphesi olanhastalardan izole edilen Candida suşlarınınörneklerinin tanımlanmasının değerlendirilmesidir.Kasım 2007 Aralık 2008 ayları arasında sistemikkandidoz hastalarından toplanan 48 farklı örnek(bronkoalveolar lavaj, beyin omurilik sıvısı, sterilvücut sıvısı, doku ve kan) çalışmaya dahil edildi.Morfolojik ve biyokimyasal testlerle Candida cinsiolarak tanımlanan mayaların kromojenik besiyeri(CHROMagar ID Candida, Biomerieux, Fransa)ekimi yapıldı. İzole edilen toplam 48 Candida suşları,morfolojik ve biyokimyasal yöntemler kullanılarak22’si C. albicans, 5’i C. keyfr, 6’sı C. tropicalis, 7’isiC. glabrata, 6’sı C. parapisilosis, biri C. krusei vebiri de C. rugosa olarak tanımlandı. Kromojenikbesiyerinde 22 C.albicans, 13 C. tropicalis/C. keyfrve 15’i diğer Candida türleri olarak tanımlandı. İkiC. parapisilosis suşu hatalı olarak tanımlandı. Sonuçolarak, kromojenik besiyerinin,konvansiyonelyöntemlerle birlikte Candida türlerinin erkentanısında faydalı bir yöntem olduğu kanaatinevarıldı.

The evaluation of chromogenic medium in ıdentification of candida species

Yeasts can often be confused owing to similarities of their morphological, biochemical and physiological properties. The aim of the study was to evaluate the identification of Candida samples by chromogenic medium which are isolated from systemic candidiasis suspected patients. Between 2007 November and 2008 December, 48 different samples (bronchoalveolar lavage, cerebrospinal fluid, sterile body fluids, tissue, blood) recovered from systemic candidiasis suspected patients have been included in this study. Isolated yeasts identified as Candida with morphological and biochemical tests were inoculated on to chromogenic medium (CHROMagar ID Candida, Biomeriux, France). A total number of 48 Candida isolates were identified as 22 C. albicans, 5 C. keyfr, 1 C. rugosa, 6 C. tropicalis, 7 C. glabrata, 6 C. parapisilosis, 1 C. krusei with morphological and biochemical methods. In chromogenic medium, results were 22 C. albicans, 13 C. tropicalis/C.keyfr and 15 other Candida species. Two C. parapsilosis isolates were identified as incorrect. In conclusion; it is suggested that chromogenic medium with conventional methods is a useful method in early diagnosis of Candida species.

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