Investigation of effect of probiotic use on organism behaviours in Drosophila melanogaster
Investigation of effect of probiotic use on organism behaviours in Drosophila melanogaster
Microbiota; Microbiome refers to the genes carried by microorganisms living commensally with humans. Recent studies have shown that the gut microbiota plays an important role in shaping the health and fitness of animals, including humans. Probiotics, known as live microorganisms that, when taken in sufficient quantities, are beneficial to the health of the organism they inhabit, have been used traditionally by people for centuries in different ways. In this study, it was aimed to investigate the effect of modified gut microbiota of Drosophila melanogaster individuals fed on probiotic supplemented media and standard media on mate selection, which is one of the behavioral characteristics. For this purpose, probiotic food containing 11 different bacterial species was added to the medium of D. melanogaster for 6 generations to reshape the fly microbiota. Flies grown on standard Drosophila medium and flies fed probiotic food were placed on the cell plates as 1 male and 1 female mixed. The mate choices of individuals carrying different markers were tracked and recorded. Obtained results were evaluated statistically. As a result, it was observed that nutrition, and therefore microbiota, was effective in the selection of mates of flies mixed in cell plates. In particular, it was determined that individuals fed with probiotics chose the opposite sex fed with probiotics in the selection of mates for mating. It has been concluded that the effects of microbiota, the importance of which has been better understood in recent years, on living things should be clarified by different studies.
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- Alp, G., Aslim, B. (2009). The importance of bifidobacteria as probiotics in the human intestinal
tract. Anadolu University Journal of Science and Technology 10(2): 343-354.
- Ayar, A. , Altun Çolak, D. , Uysal, H. & Fidan, M. (2021). Lifespan in Drosophila melanogaster
Feeding with White Tea. Erzincan University Journal of Science and Technology , 14 (2) , 582-
594.
- Borre, Y.E, O’keeffe G.W., Clarke, G., Stanton, C., Dinan, T.G., Cryan, J.F. (2014). Microbiota and
neurodevelopmental windows: implications for brain disorders. Trends Mol Med, 20:509-518.
- Deusch, S., Tilocca, B., Camarinha-Silva, A., Seifert, J. (2015). News in livestock research - use of
Omics-technologies to study the microbiota in the gastrointestinal tract of farm animals.
Computation Struct Biotechnol J, 13, 55–63.
- Dodd, D. M., Powell, J. R. (1985). Founder‐flush speciation: an update of experimental results with
Drosophila. Evolution, 39(6), 1388-1392.
- Douglas, A. E. (2018). The Drosophila model for microbiome research. Lab Animal, 47(6), 157.
Fidan, M., Ayar, A., & Konar, V. (2020). Effects of Perga (Bee bread) on Metamorphosis, Mortality,
and Fecundity in Drosophila melanogaster. Sabuncuoglu Serefeddin Health Sciences, 2(2), 1-
15.
- Fidan, M., & Ayar, A. (2021a). Genetically Modified Organisms and Effects on Human Health.
Research & Reviews in Health Sciences-I, 901.
- Fidan, M., and Ayar, A. (2021b). Comparison of The Effects of Different Forms of Green Tea on
Fecundity in Drosophila Melanogaster. Sabuncuoglu Serefeddin Health Sciences, 3(2), 26-40.
- Fidan, M., & Ayar, A. (2021c). Modulatory effects of white tea (Camellia sinensis L.) on genotoxicity
in streptozotocin and cyclophosphamide-treated Drosophila melanogaster. Progress in
Nutrition, 23(3).
- Fidan, M., & Ayar, A. (2022). The Effect of Walnut Milk on Vital Parameters and Life Span. Health & Science 2022-Ii, 151.
- Fond, G., Boukouaci, W., Chevalier, G., Regnault, A., Eberl, G., Hamdani, N. (2014). The
‘‘psychomicrobiotic’’: Targeting microbiota in major psychiatric disorders: A systematic
review. Pathol Biol (Paris), Epub ahead of print.
- Hanning, I, Diaz-Sanchez, S. (2015). The functionality of the gastrointestinal microbiome in nonhuman animals. Microbiome, 3, 51.
- Julian, R. (2014). Marchesi, The Human Microbiota and Microbiome, 124-35.
- Levy, M., Blacher, E., Elinav, E. (2017). Microbiome, metabolites and host immunity. Curr Opin
Microbiol, 35, 8-15.
- Oelschlaeger, T.A. Definition of “good bacteria” origin, effects and effective components. Online:
http://www.dgmim.de/fileadmin/CONTENT/OElschla eger_good_bacteria_vortrag.pdf,
2011, Erişim Tarihi: 04.03.2012
- Rosenberg, E., Zilber-Rosenberg I. (2011). Symbiosis and development: the hologenome concept.
Birth Defects Res C Embryo Today, 93: 56-66.
- Sharon, G., Segal, D., Ringo, J.M., Hefetz, A., Zilber-Rosenberg I. and Rosenberg E. (2010).
Commensal bacteria play a role in mating preference of Drosophila melanogaster. Proceedings
of the National Academy of Sciences of the United States of America, 107, 20051–56.
- Shin, S.C., Kim, S.H., You, H., Kim, B., Kim, A.C., Lee, K.A. (2011). Drosophila microbiome modulates
host developmental and metabolic homeostasis via insulin signaling. Science, 334, 670–74.
- van den Berg, M. J, Thomas, G., Hendriks, H., van Delden, W. (1984). A reexamination of the
negative assortative mating phenomenon and its underlying mechanism in Drosophila
melanogaster. Behav Genet 14:45–61.
- Wong, C.N., Ng, P., Douglas, A.E. (2011). Lowdiversity bacterial community in the gut of the fruitfly
Drosophila melanogaster. Environmental Microbiology, 13, 1889–1900.
- Woyke, T., Teeling H., Ivanova N.N., Huntemann M., Richter M., Gloeckner F.O., et al. (2006).
Symbiosis insights through metagenomic analysis of a microbial consortium. Nature; 443,
950–55.
- Yılmaz, K., and Altındiş, M. (2017). Digestive System Microbiota and Fecal Taransplantation,
Nobel Medicus Journal, 13(1).
Zhu, X., Han, Y., Du, J., Liu, R., Jin, K., & Yi, W. (2017). Microbiota-gut-brain axis and the central
nervous system. Oncotarget, 8(32), 53829