Emine KEMİKLİOGLU, Ebru TUNCGOVDE

Sıvı Kristaller Kullanılarak Metisiline dirençli Staphyloccoccus aureus Bakterisinin Tespiti

Metisiline dirençli Staphylococcus aureus (MRSA), halk sağlığı için temel bir patojendir ve bu bakteri genellikle insanlarda ciddi enfeksiyonlara neden olur. Son yıllarda MRSA bakterileri, zaman alıcı ve emek yoğun olan bakteri kültür ve nükleik asit esaslı yöntemlerle tespit edilmektedir. Bu çalışmada, bu sınırlamaların üstesinden gelmek için yeni bir sıvı kristal tabanlı biyoalgılama sistemi geliştirilmiştir. Bu çalışmanın birincil amacı, izotonik su ve fosfat tamponlu salin (PBS) içinde hazırlanan MRSA bakterilerinin varlığını tespit etmekti. Bu sistemde, MRSA bakterilerinin dimetiloktadesil [3- (trimetoksisilil) propil] amonyum klorür (DMOAP) kaplı yüzeye bağlanması, LC'lerin oryantasyonunu bozarak homeotropik bir yönelimden rasgele olana geçişi tetikledi. LC'lerin yönelimindeki bu geçiş, polarize ışık mikroskobu (POM) altında karanlık bir optik LC görüntüsünden parlak bir görünüme bir değişim olarak gözlemlendi ve LC moleküllerinin yansıma değerleri bir spektrometre kullanılarak belirlendi. Bu algılama mekanizması sayesinde, izotonik su içerisinde hazırlanan MRSA bakterileri 9,2x103 CFU/mL ila 9, x107 CFU/mL konsantrasyon aralığında tespit edildi. Ayrıca bu sistem kullanılarak 7.1x104 CFU/mL ile 7.1x108 CFU/mL konsantrasyon aralığında PBS'de hazırlanan MRSA bakterileri tespit edildi.

Anahtar Kelimeler:

Biyosensör, MRSA bakterileri, Sıvı kristal

Detection of Methicillin-Resistant Staphylococcus Aureus Bacteria Using Liquid Crystals

Methicillin-resistant Staphylococcus aureus (MRSA) is an essential pathogen for public health and this bacteria commonly cause serious infectious in humans. In recent years, MRSA bacteria are detected by the bacterial culture and nucleic acid-based methods which are time-consuming and labor-intensive. In this study, a novel liquid crystal (LC)-based biosensing system was developed to overcome these limitations. The objective of this study was to detect the presence of MRSA bacteria which prepared within the isotonic water and phosphate buffer saline (PBS). In this system, the binding of MRSA bacteria to the dimethyloctadecyl [3-(trimethoxysilyl) propyl] ammonium chloride (DMOAP)-coated surface disrupted the orientation of LCs, triggering a transition from a homeotropic orientation to a random one. This transition in the orientation of the LCs was observed as a change from a dark optical LC image to a bright one under a polarized light microscope (POM) and the reflection values of LC molecules were determined by using a spectrometer. Through this sensing mechanism, MRSA bacteria prepared within the isotonic water was detected ranging from the 9.2x103 CFU/mL to 9.2x107 CFU/mL concentration. Furthermore, MRSA bacteria prepared in PBS was detected in the concentration range of 7.1x104 CFU/mL to 7.1x108 CFU/mL by using this system.

Keywords:

Biosensor, MRSA bacteria, Liquid crystal,

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