The Effect of Fisetin on KATO-III cell proliferation and invasion
The Effect of Fisetin on KATO-III cell proliferation and invasion
Fisetin is a natural chemical that widely found in different plants. The aim of this study is to investigate the role of fisetin on proliferation and invasion on KATO-III human gastric carcinoma cell. KATO-III cells were seeded into the plates and 24h after dose-dependent effects of fisetin (50 and 100µM) were tested on cultured KATO-III cells. Real-time cell proliferation impedance analysis was performed up to 72h and the Transwell insert assay was performed. Relative mRNA expressions of MMP2 and MMP9, which are markers of invasion, were measured. Data are presented as fold-change in expression of any group compared to that of control group, using the 2-ΔΔCt method. Statistical comparisons were made using oneway ANOVA followed by Tukey's test. Fisetin exert anti-proliferative and anti-invasion effect on KATO-III cells. According to the cell proliferation impedance results, it was shown that proliferations of all fisetin treated groups were suppressed in dose dependent manner. According to transwell invasion results, 100µM fisetin group showed important inhibitory effects on invasion of KATO-III cells compared to the Control group. Regarding to the mRNA expression results of MMP2 and MMP9, it was shown that 50 and 100µM fisetin treatments significantly decreased these expressions compared to Control group. We conclude that fisetin negatively regulates the invasion and proliferation potentials of KATO-III cells via the MMP9 and MMP2 suppression. Thus, our findings implicate fisetin a potential therapeutic target in KATO-III cells.
Keywords:
Fisetin, invasion KATO-III, proliferation,
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- Başlangıç: 2021
- Yayıncı: Atatürk Üniversitesi