Ali YARAŞ, Veyis SELEN, Dursun ÖZER

SYNERGISTIC EFFECTS OF AGRO-INDUSTRIAL WASTES ON ALPHA AMYLASE PRODUCTION BY BACILLUS AMYLOLIQUEFACIENS IN SEMI SOLID SUBSTRATE FERMENTATION

Bu çalışma; Bacillus amyloliquefaciens NRRL B-645 ile substrat olarak soya küspesi (SM), buğday kepeği (WB) ve peynir altı suyu kombinasyonları kullanılarak ?-amilaz üretimi ile ilgilidir. Deneyler sonucunda en yüksek enzim aktivitesi SM 20g/l, WB 5g/l, peynir altı suyu 5% (v/v), peptone 1 g/l, yeast ekstrakt (YE) 0.5 g/l ve (NH4)2SO4 1 g/l konsantrasyonunda 48 saat sonunda, 33 °C fermantasyon sıcaklığı, 150 devir/dk karıştırma hızında 4257 U/ml olarak elde edildi. ?-amilaz üretimi için kullanılan bu yeni proses, farklı tarımsal atıklar kullanılarak farklı enzimlerin üretiminde de kullanılabilir

YARI KATI HAL FERMENTASYONUNDA BACILLUS AMYLOLIQUEFACIENS İLE ALFA AMİLAZ ÜRETİMİNE TARIMSAL ATIKLARIN SİNERJİK ETKİLERİ

This study concerns with the combinations of soybean meal (SM), wheat bran (WB) and whey were utilized as the substrates for ?-amylase production by Bacillus amyloliquefaciens NRRL B-645. As a result of the experiments, the highest ?-amylase activity (4257 U/ml) was obtained containing SM 20 g/l, WB 5 g/l, whey 5% (v/v), peptone 1 g/l, yeast extract (YE) 0.5 g/l and (NH4)2SO4 1 g/l at 33 °C and 150 rpm for 48 h. This innovative process for the ?-amylase production can be extended to different enzymes using various agro-industrial wastes

PDF

___

Burhan A, Nisa U, Gokhan C, Omer C, Ashabil A, Osman, G. "Enzymatic Properties of a Novel Thermostable, Thermophilic, Alkaline and Chelator Resistant Amylase From an Alkaliphilic Bacillus sp Isolate ANT-6". Process Biochemistry, 38(10), 1397-1403, 2003.
Torrado A, Vazquez J, Prieto M, Fucinos P, Montemayor M, Pastrana L, Gonzalez M, Murado M. "Amylase Production by Aspergillus oryzae in a Solid-State Bioreactor with Fed-Batch Operation using Mussel Processing Waste Waters as Feeding Medium". Journal of Chemical Technology and Biotechnology, 88(2), 226-236. 2013.
Bessler C, Schmitt J, Maurer KH, Schmid RD. "Directed Evolution of a Bacterial ?-amylase: Toward Enhanced pH-Performance and Higher Specific Activity". Protein Science, 12(10), 2141-2149, 2003.
Rodriguez CS, Rodriguez R, Gallego PP, Sanroman A. "Biodegradation of Ligninolytic Enzyme Production by Phanerochaete Chrysosporium during Semi-Solid-State Cultivation". Acta Biotechnologica, 23(1), 65-74, 2003. Stems and and Technology
Biotechnology, 83(7),
Hashemi M, Razavi SH, Shojaosadati SA, Mousavi SM, Khajeh K, Safari M. "Development of a Solid-State Fermentation Process for Production of an Alpha Amylase with Potentially Interesting Properties". Journal of Bioscience and Bioengineering, 110(3), 333-337, 2010.
Karatas H, Uyar F, Tolan V, Baysal Z. "Optimization and Enhanced Production of ?-amylase and Protease by a Newly Isolated Bacillus licheniformis ZB-05 under Solid-State Fermentation". Annals of Microbiology, 63(1), 45-52, 2013.
Sukumprasertsri M, Unrean P, Pimsamarn J, Kitsubun P, Tongta A. "Fuzzy Logic Control of Rotating Drum Bioreactor for Improved Production of Amylase and Protease Enzymes by Aspergillus oryzae in Solid-State Fermentation". Journal of Microbiology and Biotechnology, 23(3), 335-342, 2013.
Anderson E, Johanson AC, Hogerdal BH. "?-amylase Production in Aqueous two Phase Systems with Bacillus subtilis". Enzyme and Microbial Technology, 7(7), 333-338, 1985.
Tanyildizi MS, Özer D, Elibol M. "Optimization of ?-amylase Production by Bacillus sp. using Response Surface Methodology". Process Biochemistry, 40(7), 2291-2296, 2005.
Boesel LF, Azevedo HS, Reis RL. "Incorporation of Alpha-amylase Enzyme and a Bioactive Filler into Hydrophilic, Partially Degradable and Bioactive Cements (HDBCs) as a New Approach to Tailor Simultaneously Their Biomacromolecules, 7(9), 2600-2609, 2006. and Degradation Bioactive Behavior".
Pedersen H, Nielsen J. "The Influence of Nitrogen Sources on the ?-amylase Productivity of Aspergillus oryzae in Continuous Biotechnology, 53(3), 278-281, 2000. Applied Microbiology.
Pandey A. "Improvement in Solid-State Fermentation for Glucoamylase Production". Biological Wastes, 34(1), 11-19, 1990.
Sodhi HK, Sharma K, Gupta JK, Soni SK. "Production of a Thermostable ?-amylase from Bacillus sp. PS-7 by Solid State Fermentation and its Synergistic use in the hydrolysis of Malt Starch for Alcohol Production". Process Biochemistry, 40(2), 525-534, 2005.
Tanyildizi MS, Özer D, Elibol M. "Production of Bacterial ?-amylase by B. amyloliquefaciens under Solid Substrate Fermentation". Biochemical Engineering Journal, 37(3), 294-297, 2007.
Syu MJ, Chen YH. "A Study on the ?-amylase Fermentation Performed by Bacillus amyloliquefaciens". Chemical Engineering Journal, 65(3), 237-247, 1997.
Akcan N, Serin B, Uyar F. "Production and Optimization Parameters of Amylases from Bacillus subtilis RSKK96 under Solid State Fermentation". Chemical and Biochemical Engineering Quarterly, 26(3), 233-239, 2012.
Anto H, Trivedi U, Patel K. "?-amylase Production by Bacillus Fermentation". Food Technology Biotechnology, 44(2) 241-245, 2006.