Musa TURGUT, Halil KOCAMAZ, Esen AVCI, Mutlu YAKA, Gülçin ABBAN METE

Kaempferol, kurkumin ve kaempferol-kurkumin kombinasyonunun deneysel akut pankreatitteki yararlı etkileri

Amaç: Akut Pankreatit ani başlayan karın ağrısı ile kendini gösterip sistemik inflamatuar yanıta kadar ilerleyebilen acil bir klinik durumdur. Standart bir tedavisi olmayan bu hastalıkta güncel tedavi yaklaşımlarına gereksinim vardır. Deneysel akut pankreatitte kaempferol, kurkumin ve kaempferol-kurkumin kombinasyonunun etkilerini göstermeyi amaçladık. Gereç ve yöntem: Wistar albino erişkin ratlar altı gruba ayrıldı. Gruplara sırasıyla, intraperitoneal (i.p.) olarak (grup I) fizyolojik su, (grup II) L-arjinin, (grup III) Dimetil sülfoksit (DMSO), (grup IV) L-Arginin ve kurkumin, (grup V) L-Arginin ve kaempferol ile (grup VI) L-Arginin, kaempferol-kurkumin kombinasyonu uygulandı. Deney bitiminde kan ve pankreas örnekleri alındı. Histolojik, biyokimyasal ve immunohistokimyasal incelemeler yapıldı. Amilaz, lipaz, malondialdehid, TNF-α, IL-6, Superoksid dismutaz (SOD) düzeyleri ölçüldü. Pankreas doku örneklerine, pankreatit skorlaması ile NF (nükleer faktör)- κB immunohistokimya analizi yapıldı. Bulgular: Malondialdehid, tümör nekrozis faktör-alfa (TNF-α) ve interlökin-6 (IL-6) düzeyleri gruplar arasında farklı değildi. SOD düzeyleri grup VI’da diğer gruplara göre yüksekti ancak istatistiksel olarak anlamlı değildi. Ödem skoru grup VI’da grup II’ye göre daha düşük idi. Grup V ve grup VI’da inflamasyon skoru grup II’ den daha az idi. Nekroz skoru grup IV, grup V ve grup VI’da grup II’den daha az idi. Grup II’deki NF-κB boyanma skoru tedavi gruplarından farklı değildi. Sonuç: L-arjinin ile oluşturulan deneysel akut pankreatitde kaempferolün ve kaempferol-kurkumin kombinasyonunun özellikle histopatolojik olarak inflamasyonu ve nekrozu azalttığı bu sayede koruyucu etkileri olduğu görülmüştür. Anahtar kelimeler: A

The ameliorative effects of kaempferol, curcumin and kaempferol-curcumin combination on experimental acute pancreatitis

abdominal pain and may progress systemic inflammatory response syndrome. Novel treatment modalities are required for this disorder which does not have standard treatment regime. We aimed to evaluate the effects of kaempferol, curcumin and kaempferol-curcumin combination on experimental acute pancreatitis. Material and method: Wistar albino adult rats were divided into 6 groups. The groups were exposured following substances by intraperitoneal (ip) administration: (Group I) saline, (Group II) L-arginine, (Group III) Dimethyl sulphoxide (DMSO), (Group IV)L-arginine and curcumin, (Group V) L-arginine and kaempferol, (Group VI) L-arginine and curcumin-kaempferol combination. Blood and pancreas sample of the rats were obtained. Histologic, biochemical and immunohistochemical analysis were made on the samples. Amylase, lipase, tumor necrosis factor-alpha (TNF-α), interleukine-6 (IL-6), and superoxide dismutase (SOD) levels were analyzed. Pancreatitis staging and NF (nuclear factor)- κB staining were evaluated on pancreas tissues. Results: Malondialdehyde and IL-6 levels were not different between groups. Superoxide dismutase (SOD) levels were higher than other groups but it was not statistically significant. Edema score on pancreas tissue were lower than group II in group VI. Inflammatıon score were lower than group II in groups V and VI. NF (nuclear factor)- κB staining score were not different between groups. Conclusion: Kaempferol and kaempferol-curcumin combination have protective effects on L-arginine induced experimental acute pancreatitis by diminishing inflammation and necrosis.

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