Muhammed Fatih DOĞAN, Mücahit SEÇME, Yasemin ŞAHİN, Osman ÇİFTÇİ

HEC-1B hücrelerinde glabridinin hücre proliferasyonu ve uzun kodlamayan RNA ekspresyonu üzerindeki etkileri

Amaç: Endometrium kanseri dünyada en sık görülen jinekolojik kanserlerden biridir. Glabridin, Glycyrrhiza glabra (meyan kökü) kökündeki ana izoflavonoidlerden biridir. Glabridin anti-proliferatif ve anti-inflamatuar gibi çeşitli farmakolojik etkilere sahiptir. Uzun kodlamayan RNA'lar (LncRNAs) çeşitli hücresel mekanizmalarda rol oynar ve bunların değişen ekspresyonu, tümör gelişimine ve ilerlemesine katkıda bulunabilir. Bu çalışmada, glabridinin LncRNAs gen ekspresyonlarına etkileri ve HEC-1B insan endometriyal kanser hücre hattının canlılığı üzerindeki etkileri araştırılmıştır. Gereç ve yöntem: Glabridin HEC-1B hücrelerine 1 uM, 10 uM, 20 uM, 40 uM, 60 uM ve 80 uM konsantrasyonlarda uygulandı. Glabridin'in HEC-1B hücre canlılığı üzerindeki etkisi MTS testi kullanılarak değerlendirildi. Çalışmamızda araştırdığımız LncRNAs olan H19, RNU43, LNC-MYC-3:1 ve ABCC5-AS1:1 ekspresyon profilleri Real time-PCR ile yöntemi ile belirlendi. Bulgular: Glabridin, zamana ve doza bağlı bir şekilde HEC-1B hücrelerinin canlılığını azalttı. Glabridinin HEC-1B hücrelerinde maksimum yarı inhibisyon konsantrasyon (IC50) dozu 24. ve 48. saatlerde sırasıyla 21.32 μM ve 13.5 μM olarak tespit edildi. Glabridin'in LNC-MYC-3:1 ve ABCC5-AS1:1 ekspresyonunu arttırırken H19 ve RNU43 ekspresyonunda önemli bir azalmaya neden olduğu tespit edilmiştir. Sonuç: Glabridin muhtemelen LncRNAs ekspresyonlarını düzenleyerek HEC-1B kanser hücrelerinin ölümünü sağlamıştır. Sonuç olarak, glabridin insan endometriyal kanserine karşı ileride kullanılabilecek etkili bir terapötik ajan olarak önemli bir potansiyel taşımaktadır.

Anahtar Kelimeler:

Glabridin, endometriyal kanser, uzun kodlamayan RNA, HEC-1B

Effects of glabridin on cell proliferation and long non-coding RNA expression in HEC-1B cells

Purpose: Endometrial cancer is one of the most common gynecological cancers in the world. Glabridin is a main isoflavone in Glycyrrhiza glabra (licorice) root. It has several therapeutic effects such as anti-proliferative and anti-inflammatory. Long non-coding RNAs (LncRNAs) play a role in a variety of cellular processes, and their abnormal expression may contribute to tumor development and progression. In this study, the effects of glabridin on LncRNAs gene expression and viability of HEC-1B human endometrial cancer cell lines have been investigated. Materials and methods: Glabridin was applied to HEC-1B cells in concentrations of 1 μM, 10 μM, 20 μM, 40 μM, 60 μM, and 80 μM. Glabridin's effect on HEC-1B cell proliferation was also evaluated using MTS assay. Expression profiles of LncRNAs such as H19, RNU43, LNC-MYC-3:1 and ABCC5-AS1:1 were determined by real-time PCR. Results: Glabridin reduced the viability of HEC-1B cells in a time- and dose-dependent manner. The half maximal inhibitory concentration (IC50) dose in HEC-1B cells was detected to be 21.32 μM and 13.5 μM at the 24th and 48 hours, respectively. Glabridin has been observed to cause a significant decrease in the expression of H19 and RNU43 while increasing in the expression of LNC-MYC-3:1 and ABCC5-AS1:1. Conclusion: Glabridin could induce HEC-1B cell death by regulating LncRNAs expression. As a result, glabridin is a potential candidate for a more effective therapeutic agent against human endometrial cancer.

Keywords:

Glabridin, endometrial cancer, long non-coding RNA, HEC-1B,

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