Can leflunomide prevent methotrexate induced liver toxicity?

Purpose: Long-term clinical use of methotrexate is connected with a raised risk of liver injury and fibrosis. Leflunomide is a disease-modifying drug. Leflunomide has a powerful inhibitory effect on nuclear factor kappa B activation. Leflunomide also presents antioxidant activity. In this experimental study, we aimed to investigate the effects of leflunomide treatment on methotrexate -induced hepatotoxicity. Materials and methods: Thirty-nine rats were divided into 4 groups. A single dose of 20mg/kg methotrexate was injected intraperitoneally for methotrexate-induced hepatotoxicity. After induction, leflunomide (10 mg/kg) was administered into the stomach for consecutive 5 days. Then, serum samples and homogenated liver tissues were collected for analyzed serum alanine aminotransferase, alkaline phosphatase, superoxide dismutase activity, myeloperoxidase activity, glutathione levels and assessment of histopathology. Results: Leflunomide treatment significantly ameliorated total histopathologic score according to semiquantitative scale compared to the untreated group, (Pathological score 1.1+0.7 versus 5.1+2 respectively, p

Leflunomid methotrexat'a bağlı karaciğer toksisitesini önleyebilir mi?

Amaç:Uzun dönem methotrexat kullanımı artmış karaciğer hasarı ve fibrozis riski ile ilişkilidir. Leflunomid hastalık modifiye edici bir ilaçtır. Leflunomid nükleer faktör kappa B aktivasyonunun güçlü bir inhibitörüdür. Aynı zamanda anti-oksidan aktivitesi de vardır. Bu deneysel çalışmada methotrexat'ın neden olduğu karaciğer toksisitesinde leflunomid tedavisinin etkinliği araştırılmıştır. Gereç ve yöntem: 39 rat 4 gruba ayrılmıştır. Methotrexat'a bağlı karaciğer toksisitesi tek doz 20mg/kg methotrexat'ın periton içine injeksiyonu ile oluşturulmuştur. Ardından leflunomid 5 gün boyunca 10 mg/ kg dozda verilmiştir. Ardından serum örnekleri ve homojenize karaciğer örnekleri toplanmıştır. Serum alanin aminotransferaz, alkalin fosfataz, superoxide dismutaz, myeloperoxidaz aktivitesi, glutatyon düzeyleri çalışılmış ve histopatolojik değerlendirme yapılmıştır. Bulgular: Leflunomid tedavisi tedavi almayan gruba göre karaciğer semikantitatif skalaya bağlı histopatolojik değerlendirmede anlamlı düzelme sağlamıştır (Patolojik skor 1.1+0.7 ve 5.1+2, p

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Kaynak Göster

  • ISSN: 1309-9833
  • Yayın Aralığı: Yılda 4 Sayı
  • Başlangıç: 2008

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