Purpose: The aim of this study was to investigate the acute effects of a single bout of upper body wingate anaerobic power and capacity test (WAnT) on hemorheology (erythrocyte deformability, aggregation) and total oxidant/antioxidant status (TOS/TAS) in untrained individuals. Materials and methods: Fourteen sedentary healthy men ( mean age 21.86±0.55 years) performed upper body 30 s Wingate Test. Blood samples were obtained before and immediately after the exercise within 1 minute. Hemorheological parameters were determined by an ektacytometer. TOS/TAS were measured using a commercial kit and blood lactate concentration was determined by a lactate analyzer. Results: There were statistically significant decrements in RBC deformability determined at 9 shear stresses between 0.3 and 30 Pa (p0.05). Conclusion: These data show that one session of upper body WAnT has acute effects on circulation by reducing red blood cell (RBC) deformability and increasing erythrocyte aggregation. Altered hemorheological parameters may be explained by the oxidative stress enhancing effect of exercise.