Zulaiha Gidado MUKHTAR, Dursun ÖZER, Fikret KARATAŞ, Sinan SAYDAM

Türkiye ve Nijerya’dan Patlıcan Türlerinde Bazı Biyokimyasal Parametre İçeriklerinin Araştırılması

Bu çalışmada, Türkiye (koyu ve açık renkli patlıcan) ve Nijerya'da (white garden egg, bitter apple ve bitter tomato) yetişen patlıcan örneklerinin likopen, beta karoten, vitaminler, glutatyon (GSH, GSSG) malondialdehit (MDA), 4-OH neoneal (HNE) içerikleri HPLC ile belirlenmiştir. Ayrıca toplam fenolik ve flavonoid madde miktarları ve antioksidan kapasitenin (ABTS, IC50) belirlenmesinde ise spektrofotometre kullanılmıştır. Vitamin A, E, β-karoten ve likopen miktarları sırasıyla 0,13-3,63; 3,63-39,0; 1,87-30,5; 1,52-6,79 µg/g dw arasında değişmektedir. Aynı şekilde C, B1, B2, B3, B5, B6, B9 ve B12 vitamin miktarları ise sırasıyla 357-1136; 11,0-95,6; 1,9-5,4; 83-265; 30,16-65,43; 127-348; 24,74-78,6; 0,11-0,68 µg/g dw arasında değişmektedir. GHS, GSSG, MDA ve 4-HNE miktarları ise sırasıyla 364-1930; 225-962; 1,5-8,4; 24,57-38,25 µg/g dw arasında bulunmuştur. Total fenolik madde 706-1260 µg GAE/g dw arasında iken, toplam flavonoid madde miktarı 167-356 µg QE/g dw arasında bulunmuştur. ABTS değerleri 365-692 µmol Troloxs/g dw arasında değişirken, IC50 değerleri 65,1-99,3 µg/mL arasında bulunmuştur. Hem Türkiye'de hem de Nijerya'da yetiştirilen patlıcan türlerinde yapılan araştırma sonucunda gözlenen farklılıklar, genetik yapıları ve coğrafi kökenlerinden kaynaklandığı söylenebilir.

Anahtar Kelimeler:

Antioksidan kapasitesi, Fenolik madde, Glutatyon, Patlıcan, Vitaminler

Some Biochemical Parameters of Eggplant Species from Turkey and Nigeria

In this study, the amounts of vitamins, beta-carotene, lycopene, glutathione (GSH, GSSG), malondialdehyde (MDA), and hydroxyneoneal (4-HNE) in eggplant samples grown in Turkey (dark and light colored eggplant) and Nigeria (white garden egg, bitter apple and bitter tomato) were determined by HPLC. In addition, the total amount of phenolic, and flavonoid substances and antioxidant capacity (ABTS, IC50) were determined by a UV-Visible spectrophotometer. The amounts of vitamins A, E, β-carotene and lycopene were found to be in the ranged of 0.13 -3.63; 3.63-39.0; 1.87-30.5; 1.52-6.79 µg/g dw, respectively. The amounts of vitamin C, B1, B2, B3, B5, B6, B9 and B12 ranged 357-1136; 11.0-95.6; 1.9-5.4; 83-265; 30.16-65.43; 127-348; 24.74-78.6; 0.11-0.68 µg/g dw, respectively. GHS, GSSG, MDA and 4-HNE were found to be in between 364-1930; 225-962; 1.5-8.4; 24.57-38.25 µg/g dw, respectively. While the total phenolic substance was between 706-1260 µg GAE/g dw, the total amount of flavonoid substance was found to be in between 167-356 µg QE/g dw. ABTS values ranged between 365-692 µmol Troloxs/g dw, while IC50 values were in 65.1-99.3 µg/mL. It can be said that the differences observed in the parameters observed in eggplant varieties grown both in Turkey and Nigeria are due to genetic and geographical differences.

Keywords:

Antioxidant capacity, Eggplant, Glutathione, Phenolic substance, Vitamins,

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