Emine Esin YALINBAŞ, Raziye AKCILAR, Havva KOÇAK, Murat Soner ÇİRKİNOĞLU, Mehmet METİNEREN, Harun KAÇAR

Nekrotizan Enterokolitli Yenidoğan Rat Modelinde Silimarinin Antioksidan Etkileri

Nekrotizan enterokolit (NEK), prematüre bebeklerde en sık görülen gastrointestinal problemdir. Bu çalışmanın amacı, NEK modeli oluşturulan yenidoğan sıçanlarda silimarin (SLY)'nin koruyucu ve antioksidan etkilerini değerlendirmektir. Yirmi sekiz Sprague-Dawley sıçanı çalışmaya dahil edildi. Sıçanlar rastgele dört gruba ayrıldı: kontrol (C), C+SLY, NEK ve NEK+SLY. NEK, hiperosmolar enteral formül beslenmesi ile indüklendi, yavru sıçanlar hipoksi ve soğuk stresine maruz bırakıldı. Bağırsak dokusunun makroskopik skorlaması değerlendirildi ve biyokimyasal, histopatolojik inceleme için doku örnekleri alındı. Süperoksit dismutaz (SOD), glutatyon peroksidaz (GPx), nitrik oksit (NO), malondialdehit (MDA), total antioksidan status (TAS), total oksidan status (TOS) ve oksidatif stres indeksi (OSI) düzeyleri biyokimyasal olarak değerlendirildi. NEK+SLY grubunda doku TAS (p = 0,007), SOD (p = 0,004) ve GPx düzeylerinde önemli artış ve NO düzeylerinde azalma vardı. NEK grubunda doku MDA, TOS (p = 0,001) ve OSI (p = 0,001) değerleri anlamlı derecede yüksek saptandı. NEK + SLY grubundaki sıçanların bağırsak dokusu, değerlendirildiğinde NEC grubundaki sıçanlardan daha iyi histopatolojiye sahipti. Silimarin, neonatal sıçanlarda NEK'e karşı faydalı etkilere sahiptir. SLY'nin serbest radikal düzeylerini ve oksidatif stresi azalttığı, antioksidan kapasiteyi arttırdığı ve NEK'e bağlı bağırsak hasarının şiddetini iyileştirdiği görülmektedir.

Anahtar Kelimeler:

nekrotizan enterokolit, silimarin, neonatal rat, oksidatif stres

Antioxidative Effects of Silymarin in A Neonatal Rat Model of Necrotizing Enterocolitis

Necrotizing enterocolitis (NEC) is the most common gastrointestinal problem in premature infants. The aim of this study is to evaluate the protective and antioxidant effects of silymarin (SLY) in newborn rats with NEC model. Twenty-eight Sprague-Dawley rats were included in the study. The rats were randomized into four groups: control (C), C+SLY, NEC and NEC+SLY. NEC was induced by hyperosmolar enteral formula feeding, and the pups were exposed to hypoxia and cold stress. Macroscopic scoring of the intestinal tissue was evaluated and tissue samples were obtained for biochemical, histopathological examination. Superoxide dismutase (SOD), glutathione peroxidase (GPx), nitric oxide (NO), malondialdehyde (MDA), total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) levels were biochemically evaluated. Results: In the NEC + SLY group, there was a considerable rise in tissue TAS (p = 0.007), SOD (p = 0.004) and GPx levels, as well as a decrease in NO levels. Significantly higher tissue MDA, TOS (p = 0.001) and OSI (p = 0.001) values were detected in the NEC group. The intestinal tissue of rats in the NEC + SLY group had better histopathology than rats in the NEC group when evaluated. Silymarin has beneficial effects against NEC in neonatal rat. It appears that SLY reduces free radical levels and oxidative stress, increases antioxidant capacity, and ameliorates the severity of intestinal damage due to NEC

Keywords:

necrotizing enterocolitis, silymarin, neonatal rats, oxidative stress,

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