Funda TURAN, Ayşegül ERGENLER, Fevzi BARDAKÇI

Monitoring DNA Damage in Suez Pufferfish (Lagocephalus suezensis) from the Northeastern Mediterranean

Industrial effluents, agricultural runoff, and municipal wastewaters contain unknown substances and complex mixtures that are released into the environment and can lead to contamination of surface and subsurface waters. Such activities have endangered the existence of ecosystems and their inhabitants. Changes in the genome caused by genotoxic agents led to mutations and pose a burden to the populations of fish species. In the present study, we have used the alkaline Comet assay to detect the genotoxicity in Suez pufferfish (Lagocephalus suezensis) sampled from two different gulfs (Iskenderun and Mersin Bay), Northeastern Mediterranean. At the end of the study, the damage frequency (%), arbitrary unit and genetic damage index (%) were evaluated in gill and liver cells of L. suezensis by comet assay. The DNA damage in the gill and liver cells of L. suezensis in the present study were observed with a higher level of DNA damage in gill cells compared to liver cells in both the Iskenderun and Mersin Bays. The highest level of DNA damage (55.01±1.02%), arbitrary unit (143.01±7.21) and genetic damage index (1.43±0.07 %) were found in gill cells of L. suezensis from Mersin Bay. Statistically significant differences were found between DNA damage, Arbitrary unit and genetic damage index values of the two locations (p

Keywords:

Lagocephalus suezensis, DNA damage comet assay, Iskenderun Bay, Mersin Bay,

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Ameur, W. B., de Lapuente, J., El Megdiche, Y., Barhoumi, B., Trabelsi, S., Camps, L., & Borràs, M. (2012). Oxidative stress, genotoxicity and histopathology biomarker responses in mullet (Mugil cephalus) and sea bass (Dicentrarchus labrax) liver from Bizerte Lagoon (Tunisia). Marine Pollution Bulletin, 64(2), 241-251. https://doi.org/10.1016/j.marpolbul.2011.11.026.
Ateeq, B., Farah, M. A., & Ahmad, W. (2005). Detection of DNA damage by alkaline single cell gel electrophoresis in 2, 4-dichlorophenoxyacetic-acid-and butachlor-exposed erythrocytes of Clarias batrachus. Ecotoxicology and Environmental Safety, 62(3), 348-354.
Anderson, M.R., Scruton, D.A., Williams, U.P., Payne, J.F. (1995). Mercury in fish in the Smallwood Reservoir, Labrador, twenty one years after impoundment. Water Air Soil Pollution, 80(1–4):927–930. https://doi.org/10.1007/BF01189746.
Bolognesi, C., Cirillo, S., & Chipman, J. K. (2019). Comet assay in ecogenotoxicology: Applications in Mytilus sp.. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 842, 50-59. https://doi.org/10.1016/j.mrgentox.2019.05.004.
Cavalcante, D.G.S.M., Martinez, C.B.R., Sofia, S.H. (2008). Genotoxic effects of roundup on the fish Prochilodus lineatus. Mutation Research, 655,41–46.
Collins, A., 2004. The comet assay for DNA damage and repair. Molecular Biotechnology 26, 249–261. https://doi.org/10.1385/MB:26:3:249.
Cui, Z., Luan, X., Li, S., Zhao, X., Lin, Z., Li, J., ... & Xie, J. (2021). Genotoxicity detection of oil-containing drill cuttings by Comet assay based on a demersal marine fish Mugilogobius chulae. Ecotoxicology and Environmental Safety, 208, 111655. https://doi.org/10.1016/j.ecoenv.2020.111655.
Doğdu, S. A., Uyan, A., Uygur, N., Gürlek, M., Ergüden, D., & Turan, C. (2016). First record of the Indo-Pacific striped eel catfish, Plotosus lineatus (Thunberg, 1787) from Turkish marine waters. Natural and Engineering Sciences, 1(2), 25-32. https://doi.org/10.28978/nesciences.286245.
Doğdu, S. A., Turan, C., Depci, T., & Ayas, D. (2021). Natural hydroxyapatite obtained from pufferfish teeth for potential dental application. Journal of Ceramic Processing Research, 22(3), 356-361. https://doi.org/10.36410/jcpr.2021.22.3.356.
Ergüden, D., Gurlek, M., Uygur, N., & Turan, C. (2013). Occurrence of fangtooth moray Enchelycore anatina (Lowe, 1839)(Muraenidae) in Iskenderun Bay, Eastern Mediterranean, Turkey. Biharean Biologist, 7(2), 108-110.
Gürlek, M., Ergüden, D., Uyan, A., Doğdu, S. A., Yağlıoğlu, D., Öztürk, B., & Turan, C. (2016). First record red lionfish Pterois volitans (Linnaeus, 1785) in the Mediterranean Sea. Natural and Engineering Sciences, 1(3), 27-32. https://doi.org/10.28978/nesciences.286308.
Kaur, P., Dhull, S. B., Sandhu, K. S., Salar, R. K., & Purewal, S. S. (2018). Tulsi (Ocimum tenuiflorum) seeds: in vitro DNA damage protection, bioactive compounds and antioxidant potential. Journal of Food Measurement and Characterization, 12(3), 1530-1538.
Kukla, S. P., Slobodskova, V. V., Zhuravel, E. V., Mazur, A. A., & Chelomin, V. P. (2022). Exposure of adult sand dollars (Scaphechinus mirabilis)(Agassiz, 1864) to copper oxide nanoparticles induces gamete DNA damage. Environmental Science and Pollution Research, 29(26), 39451-39460. https://doi.org/10.1007/s11356-021-18318-0.
Mayer, C., Popanda, O., Zelezny, O., von Brevern, M. C., Bach, A., Bartsch, H., & Schmezer, P. (2002). DNA repair capacity after γ-irradiation and expression profiles of DNA repair genes in resting and proliferating human peripheral blood lymphocytes. DNA Repair, 1(3), 237-250. https://doi.org/10.1016/S1568-7864(01)00019-2.
Pitarque, M., Creus, A., Marcos, R., Hughes, J. A., & Anderson, D. (1999). Examination of various biomarkers measuring genotoxic endpoints from Barcelona airport personnel. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 440(2), 195-204.
Sarker, S., Vashistha, D., Saha Sarker, M., and Sarkar, A., (2018). DNA damage in marine rock oyster (Saccostrea Cucullata) exposed to environmentally available PAHs and heavy metals along the Arabian Sea coast. Ecotoxicology and Environmental Safety, 151: 132-143.
Singh, N.P., McCoy, M.T., Tice, R.R., Schneider, E.L. (1988) A simple technique for quantitation of low levels of DNA damage in individual cells. Experimental Cell Research, 175 (1),184–191. https://doi.org/10.1016/0014-4827(88)90265-0.
Siu, S. Y. M., Lam, P. K. S., Martin, M., Caldwell, C. W., and Richardson, B. J., 2008. The use of selected genotoxicity assays in green-lipped mussels (Perna viridis): A validation study in Hong Kong coastal water. Marine Pollution Bulletin, 57: 479-492.
Slobodskova, V. V., Zhuravel, E. V., Kukla, S. P., & Chelomin, V. P. (2019). Evaluation of DNA damage in the marine mussel Crenomytilus grayanus as a genotoxic biomarker of pollution. Journal of Ocean University of China, 18(1), 159-164.
Ternjej, I., Mihaljević, Z., Stanković, I., Kerovec, M., Sipos, L., Želježić, D., & Kopjar, N. (2010). Estimation of DNA integrity in blood cells of eastern mosquitofish (Gambusia holbrooki) inhabiting an aluminium-polluted water environment: an alkaline comet assay study. Archives Of Environmental Contamination and Toxicology, 59(2), 182-193.
Turan, C. (2020). Species distribution modelling of invasive alien species; Pterois miles for current distribution and future suitable habitats. Global Journal of Environmental Science and Management, 6(4), 429-440. https://doi.org/10.22034/gjesm.2020.04.01.
Turan, C., Ergüden, D., Gürlek, M., Yağlioğlu, D., Uyan, A., & Uygur, N. (2014). First record of the Indo-Pacific lionfish Pterois miles (Bennett, 1828)(Osteichthyes: Scorpaenidae) for the Turkish marine waters. Journal of Black Sea/Mediterranean Environment, 20(2), 158-163.
Turan, C., Gürlek, M., Ergüden, D., Uyan, A., Karan, S., & Doğdu, S. A. (2017). Assessing DNA Barcodes for Identification of Pufferfish Species (Tetraodontidae) in Turkish Marine Waters. Natural and Engineering Sciences, 2(3), 55-66. https://doi.org/10.28978/nesciences.369538.
Turan, C., Gürlek, M., Başusta, N., Uyan, A., Doğdu, S. A., & Karan, S. (2018). A checklist of the non-indigenous fishes in Turkish marine waters. Natural and Engineering Sciences, 3(3), 333-358. https://doi.org/10.28978/nesciences.468995.
Turan, F., & Ergenler, A. (2019). Assessment of DNA Damage by Comet Assay in Trachinotus ovatus Cells from Mersin Bay in the Northeastern Mediterranean. Natural and Engineering Sciences, 4(3): 25-31.
Turan, F., & Ergenler, A. (2022). Investigation of The Genotoxic Effect of Acetamiprid In Cyprinus carpio using the Micronucleus Analysis and the Comet Assay. Turkish Journal of Maritime and Marine Sciences, 1-10. https://doi.org/10.52998/trjmms.1037906.
Turan, F., Eken, M., Ozyilmaz, G., Karan, S., & Uluca, H. (2020a). Heavy metal bioaccumulation, oxidative stress and genotoxicity in African catfish Clarias gariepinus from Orontes river. Ecotoxicology, 29, 1522-1537. https://doi.org/10.1007/s10646-020-02253-w.
Turan, F., Karan, S., & Ergenler, A. (2020b). Effect of heavy metals on toxicogenetic damage of European eels Anguilla anguilla. Environmental Science and Pollution Research, 27(30), 38047-38055. https://doi.org/10.1007/s11356-020-09749-2.
Udroiu, I. (2006). Feasibility of conducting the micronucleus test in circulating erythrocytes from different mammalian species: an anatomical perspective. Environmental and Molecular Mutagenesis, 47(9), 643-646. https://doi.org/10.1002/em.20258.