Ayşenur GÜRGEN, Sana Adel TABBOUCHE, Sibel YILDIZ, Ali Osman KILIÇ

Türkiye’den Toplanan Bazı Yabani Mantarların Antimikrobiyal ve Anti-Quorum Sensing (Çoğunluğu Algılama) Aktiviteleri

Yabani mantarlar, tıbbi özellikleri nedeniyle çok eski tarihten beri bilinen, ormanların önemli bir parçasıdır. Bu çalışmada, Karadeniz bölgesinden toplanan bazı yabani mantarların (Amanita rubescens, Russula delica, Lactarius sp.) anti mikrobiyal ve anti-quorum sensing (çoğunluğu algılama) aktiviteleri incelenmiştir. Mantar ekstraktları süper kritik akışkan ekstraksiyonu metodu ile hazırlanmıştır. Ekstraktların antimikrobiyal potansiyelleri Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Enterococcus faecalis ATCC 29212, Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumoniae ATCC 13883, Proteus mirabilis ATCC 7002, Listeria monocytogenes ATCC 43251 and Candida albicans ATCC 10231 mikroorganizmalarına karşı agar kuyucuk difüzyon yöntemiyle test edilmiştir. Anti-quorum sensing (çoğunluğu algılama) aktivite ise Chromobacterium violaceum ATCC 12472 bakterisi üzerinde test edilmiştir. Sonuçlar, Amanita rubescens mantar ekstraktının Staphylococcus aureus'u inhibe ettiğini, Amanita rubescens ve Lactarius ssp. ekstraktlarının Chromobacterium violaceum a karşı anti-quorum sensing (çoğunluğu algılama) aktiviteye sahip olduğunu göstermiştir.

Anahtar Kelimeler:

Antimikrobiyal, Anti-Quorum Sensing (Çoğunluğu Algılama), Mantar, Türkiye

Antimicrobial and Anti-Quorum Sensing Activity of Some Wild Mushrooms Collected from Turkey

Wild mushrooms are an important part of forests that have been known since the early history for their excellent medicinal properties. In this study, anti-microbial and anti-quorum sensing activities of some wild mushrooms (Amanita rubescens, Russula delica, Lactarius sp.) collected from the Black Sea region in Turkey were investigated. Mushroom extracts were prepared by supercritical fluid extraction method. Antimicrobial potential of extracts was tested by agar well diffusion method against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Enterococcus faecalis ATCC 29212, Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumoniae ATCC 13883, Proteus mirabilis ATCC 7002, Listeria monocytogenes ATCC 43251 and Candida albicans ATCC 10231. Anti-quorum sensing activity was tested on Chromobacterium violaceum ATCC 12472 bacteria. The results revealed that Amanita rubescens showed an inhibitory effect against Staphylococcus aureus and Amanita rubescens and Lactarius sp. extracts showed anti-quorum sensing activity against Chromobacterium violaceum.

Keywords:

Anti-microbial, Anti-Quorum Sensing, Mushroom, Turkey,

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