KANADA’DA YETİŞEN İKİ Thuja L. (CUPRESSACEAE) TÜRÜNÜN UÇUCU YAĞ KOMPOZİSYONU

Kanada’da yetişen Thuja occidentalis L. ve Tetraclinis articulate (Vahl) Masters (synonym: Thuja articulate) türlerinin ibrelerinden elde edilen yağlar HS-SPME / GC-MS tekniği ile analiz  edildi. Sırasıyla %92.30 and %93.42’lik toplam yağ miktarında otuzbir ve ellibir bileşen tespit edildi. Thuja occidentalis’in ana bileşenleri bornilasetat (%30.00), limonen (%7.56), kamfor (%7.33), α-pinen (%7.18) ve δ-kadinen (%6.01) olarak belirlendi; Tetraclinis articulata’da ise α pinen (%32.67), 3-karen (%18.29), β-mirsen (%11.69) ve bornilasetat (%5.88) ana bileşenler olarak bulundu. Sonuçlara gore T. occidentalis’in kemotipi bornilasetat, T. articulata’ nın kemotipi α-pinen olarak belirlenmiş ve HS-SPME yöntemi tıbbi ve aromatik bitkilerin rutin kontrol ve analizlerinde kullanılabilecek bir yöntem olarak belirlenmiştir.

-

The essential oil needles of Thuja occidentalis L. and Tetraclinis articulate (Vahl) Masters (synonym: Thuja articulate) growing in Canada, were analyzed by HS-SPME / GC-MS technique. Thirty one and fifty one compounds were identified representing 92.30% and 93.42% of the oil, respectively. The main constituents of Thuja occidentalis were bornylacetate (30.00%), limonene (7.56%), camphor (7.33%), α-pinene (7.18%) and δ-cadinene (6.01%), whereas α-pinene (32.67%), 3-carene (18.29%), β-myrcene (11.69%) and bornylacetate (5.88%) were the major constituents of Tetraclinis articulata. The results showed that T. occidentalis chemotype was bornylacetate whereas α-pinene was chemotype of Tetraclinis articulate and HS-SPME method can be applied to routine control analysis of aromatic and medicinal plants

___

  • Chang, L.C., Song, L.L., Park, E.J., Luyengi L., Lee K.J., Fransworth, N.R., Pezzuto, J.M., Kinghorn A.D., Journal of Natural Product, 63, 1235-1238, 2000.
  • Millspaugh, C.F., American medicinal plants: An illustrated and descriptive guide to plants indige- nous to and naturalized in the United States are used in medicine. Dover Publications, New York, 1974.
  • Assadi, M., Flora of Iran, 11-12, 1998.
  • Chen, Y., Li, S., Yang, L., Jiang, Z., Comparative study on chemical constituents of essential
  • oils from several parts of Platycladus orientalis (L.) Franco. Linchan Huaxue Yu Gongye, 4, 1-11, 1984.
  • Li, Z., Liu, S., Chemical constitution of essential oil from the fruit of Biota orientalis (L.) Endl., Zhongguo Yaoxe Zazhi, 32, 138-139, 1997.
  • British Herbal Pharmacopoeia, Thuja, British Herbal Medicine Association,West Yorks, UK, 210-211, 1983.
  • Shimada, K., Contribution to anatomy of the central nervous system of the Japanese upon the vermal arbour vitae. Okajimas Folia Anat Jpn., 28, 207-227, 1956.
  • Naser, B., Bodinet, C., Tegtmeier, M., Lindequist, U., Thuja occidentalis (Arbor vitae): A review of its pharmaceutical, pharmacological and clinical properties. Alternatif Medical, 2, (1), 69-78, 2005.
  • Arctander St.: Perfume and Flavor Chemicals (Aroma Chemicals). Arctander Publ., Montclair, 1969.
  • Bauer, K., Garbe, D., Surburg, H., Common Fragrance and Flavour Materials, 3rd Ed., VCH, Weinheim, 1997.
  • Verzera, A., Zino, M., Condurso, C., Romeo, V., Zappala, M., Anal. Bioanal. Chemistry, 380, 930-936, 2004.
  • Van Den Dool, H., Kratz, P. D., A generalization mof the retention index system including linear temperature programmed gas-liquid partition chromatography, Journal Chromatography, 11, 463-471, 1963.
  • Nickavar, B., Amin, G., Parhami, S., Volatile Constituents of the fruit and leaf oils of Thuja orientalis L. Grown in Iran. Z. Naturforsch, 58c, 171-172, 2003.
  • Guleria, S., Kumar, A., Tiku, AK., Chemical composition and Fungitoxic Activity of Essential Oil of Thuja orientalis L. Grown in the North-Western Himalaya. Z. Naturforsch, 63c, 211-214, 2008.
  • Kılıç, O., Koçak, A.,.Essential Oil Composition of Six Pinus L. Taxa (Pinaceae) from Canada and their chemotaxonomy. Journal of Agricultural Science and Technology B 4 January, 67-73, 2014.
  • Kılıç, O., Koçak, A.,. Volatile Constituents of Juniperus communis L., Taxus Canadensis Marshall. and Tsuga canadensis (L.) Carr. From Canada, Journal of Agricultural Science and Technology B 4, January, 135-140, 2014.