IN VITRO INHIBITION OF BOVINE LIVER CARBONIC ANHYDRASE II BY SOME DRUGS

Karbonik anhidraz enzimi (EC: 4.2.1.1; CA) sığır karaciğerinden saflaştırılarak bazı antibiyotiklerin inhibisyon etkileri incelendi. Saflaştırma prosedürü, homojenatın hazırlanması ve Sepharoz 4B-Tirozin-Sulfanamid Kromatografisinden oluştu. Birbirini takip eden bu iki yöntem ileenzim 65,24 kat, % 50,86 verimle ve spesifik aktivitesi 1117.24 U/mg protein olarak saflaştırıldı. Enzim üzerine bazı antibiyotik ilaçların in vitro inhibisyon etkileri belirlendi. Bu inhibitörler için IC 50 değerleri, % aktiviteye karşı inhibitor konsantrasyon [I] grafiği çizilmek suretiyle belirlendi. Siprofloksazin ve seftazidim inhibitörlerinin IC değerleri sırasıyla 1.27 ve 5.29 mM olarak tespit 50 edildi.           Anahtar Kelimeler: Karbonik anhidraz(CA), Sığır, Karaciğer, İnhibitör, Antibiyotik

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Bibtex @ { msufbd209879, journal = {Muş Alparslan Üniversitesi Fen Bilimleri Dergisi}, issn = {2147-7930}, eissn = {2149-6455}, address = {}, publisher = {Muş Alparslan Üniversitesi}, year = {2015}, volume = {3}, number = {1}, pages = {334 - 339}, doi = {10.18586/msufbd.81196}, title = {IN VITRO INHIBITION OF BOVINE LIVER CARBONIC ANHYDRASE II BY SOME DRUGS}, key = {cite}, author = {Aygül, İmdat and Güleşçe, Nuri} }
APA Aygül, İ. & Güleşçe, N. (2015). IN VITRO INHIBITION OF BOVINE LIVER CARBONIC ANHYDRASE II BY SOME DRUGS . Muş Alparslan Üniversitesi Fen Bilimleri Dergisi , 3 (1) , 334-339 .
MLA Aygül, İ. , Güleşçe, N. "IN VITRO INHIBITION OF BOVINE LIVER CARBONIC ANHYDRASE II BY SOME DRUGS" . Muş Alparslan Üniversitesi Fen Bilimleri Dergisi 3 (2015 ): 334-339 <
Chicago Aygül, İ. , Güleşçe, N. "IN VITRO INHIBITION OF BOVINE LIVER CARBONIC ANHYDRASE II BY SOME DRUGS". Muş Alparslan Üniversitesi Fen Bilimleri Dergisi 3 (2015 ): 334-339
RIS TY - JOUR T1 - IN VITRO INHIBITION OF BOVINE LIVER CARBONIC ANHYDRASE II BY SOME DRUGS AU - İmdatAygül, NuriGüleşçe Y1 - 2015 PY - 2015 N1 - DO - T2 - Muş Alparslan Üniversitesi Fen Bilimleri Dergisi JF - Journal JO - JOR SP - 334 EP - 339 VL - 3 IS - 1 SN - 2147-7930-2149-6455 M3 - UR - Y2 - 2022 ER -
EndNote %0 Muş Alparslan Üniversitesi Fen Bilimleri Dergisi IN VITRO INHIBITION OF BOVINE LIVER CARBONIC ANHYDRASE II BY SOME DRUGS %A İmdat Aygül , Nuri Güleşçe %T IN VITRO INHIBITION OF BOVINE LIVER CARBONIC ANHYDRASE II BY SOME DRUGS %D 2015 %J Muş Alparslan Üniversitesi Fen Bilimleri Dergisi %P 2147-7930-2149-6455 %V 3 %N 1 %R %U
ISNAD Aygül, İmdat , Güleşçe, Nuri . "IN VITRO INHIBITION OF BOVINE LIVER CARBONIC ANHYDRASE II BY SOME DRUGS". Muş Alparslan Üniversitesi Fen Bilimleri Dergisi 3 / 1 (Ocak 2015): 334-339 .
AMA Aygül İ. , Güleşçe N. IN VITRO INHIBITION OF BOVINE LIVER CARBONIC ANHYDRASE II BY SOME DRUGS. MAUN Fen Bil. Dergi.. 2015; 3(1): 334-339.
Vancouver Aygül İ. , Güleşçe N. IN VITRO INHIBITION OF BOVINE LIVER CARBONIC ANHYDRASE II BY SOME DRUGS. Muş Alparslan Üniversitesi Fen Bilimleri Dergisi. 2015; 3(1): 334-339.
IEEE İ. Aygül ve N. Güleşçe , "IN VITRO INHIBITION OF BOVINE LIVER CARBONIC ANHYDRASE II BY SOME DRUGS", , c. 3, sayı. 1, ss. 334-339, Oca. 2015, doi:10.18586/msufbd.81196