INVESTIGATION OF THE EFFECT OF DIOCTYLFLUORENYL-co- BENZOTHIADIAZOLE BEARING POLYMER AND MWCNTS ON BIOSENSOR PERFORMANCE

The combination of conjugated polymers and carbon nanotubes serve great contributions in many research fields because of their superior characteristics. Herein, an electrochemical biosensor, using poly[(9,9-dioctylfluorenyl-2,7-diyl)-alt-co- (1,4-benzo-{2,1’,3}-thiadiazole)] (PFBTz) and multi-walled carbon nanotubes (MWCNTs) was constructed for glucose sensing. To create such a surface, a graphite electrode was coated with PFBTz and MWCNTs, respectively. Then, glucose oxidase (GOx) was immobilized onto this new platform using glutaraldehyde (GA) as the cross linker. The limit of detection, linear range and sensitivity values were found to be 0.018 mM, 0.025–0.6 mM and 66.0 μAmM-1cm-2, respectively. The selectivity of the prepared biosensor was also examined in the presence of interferences and as a result, it has been observed that the biosensor response was much higher for glucose in all measurements. Surface characterizations before and after enzyme immobilization were done using scanning electron microscopy (SEM) and cyclic voltammetry (CV) techniques. In the final part of the study, the constructed biosensor was applied to beverages for glucose detection and very promising results were obtained. With this work, a robust, novel and economic sensing platform was prepared for glucose determination.

DİOKTİLFLUORENİL-KO-BENZOTİYADİAZOL POLİMER VE ÇOK DUVARLI KARBON NANOTÜPÜN BİYOSENSÖR PERFORMANSI ÜZERİNE ETKİSİNİN İNCELENMESİ

Karbon nanotüp ve konjuge polimerlerin kombinasyonu çok iyi özelliklere sahip olmalarından dolayı birçok alanda güzel katkılar sağlamaktadır. Bu çalışmada, elektrokimyasal bir biyosensör 9,9-dioktilfluorenil-2,7-diyl) -alt-ko- (1,4-benzo- (2,1 ', 3} -tiyadiazol) (PFBTz) ve çok duvarlı karbon nanotüplerin (MWCNTs) kullanılması ile glikoz tayini için kullanılmıştır. Böyle bir yüzey oluşturmak için, grafit elektrot yüzeyi sırasıyla PFBTz ve MWCNTs ile modifiye edilmiştir. Daha sonra glikoz oksidaz (GOx) bu yeni platform üzerine çapraz bağlayıcı olan gluteraldehit (GA) yardımıyla immobilize edilmiştir. Tayin sınırı, lineer aralık ve hassasiyet değerleri sırasıyla 0.018 mM, 0.025–0.6 mM ve 66.0 μAmM-1cm-2 olarak bulunmuştur. Önerilen biyosensörün seçiciliği de, girişim yapan moleküllerin varlığında test edilmiştir ve biyosensör cevabının, tüm ölçümlerde glikoz için çok daha yüksek olduğu belirlenmiştir. Enzim immobilizasyonundan önce ve sonra yüzey karakterizasyonu, taramalı elektron mikroskobu (SEM) ve siklik voltametri (CV) teknikleri kullanılarak yapılmıştır. Çalışmanın son bölümünde ise, üretilen biyosensör kullanılarak içeceklerde glikoz tayini başarılı bir şekilde test edilmiştir. Bu çalışma ile glikozun tespiti için hızlı, yeni ve uygun maliyetli bir platform tasarlanmıştır.

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