Esin SAKALLI ÇETİN, Yunus ÇETİNTAŞ, Said NADEEM, Mehmet Ali ÖZLER, Elif ELİUZ ERDOĞAN

Eucalyptus camaldulensis YAPRAK EKSTRESİNDEN HAZIRLANAN AgNP’LERİN YEŞİL SENTEZİ, ANTİMİKROBİYAL VE ANTİKANSER AKTİVİTELERİ

Eucalyptus camaldulensis'in oda koşullarındaki metanol ekstresi aracılı su fraksiyonu (Ecs) kullanılarak gümüş nanopartiküllerin (EcAgNP'ler) yeşil sentezini sunuyoruz. EcAgNP'leri karakterize etmek için UV-vis, HR-TEM ve SEM-EDS cihazları kullanıldı. EcAgNP'lerin UV-visible spektrumu 420 nm'de yüzey plazmon rezonans pikini gösterdi. HR-TEM görüntüleri, EcAgNP'lerin çapının 3.7-29.6 nm aralığında küresel olduğunu gösterdi. Ag+ iyonları, başka indirgeyici madde eklenmeden Ecs’deki fitokimyasallar tarafından Ago'a indirildi. Ecs içindeki biyomoleküllerin varlığı, gallik asit (20.26±0.10 ppb) ve kuarsetin (12.4±0.08 ppb) major bileşenler olarak tespit edildiği UPLC-MS/MS ile araştırıldı. Ecs ve sentezlenmiş EcAgNP'lerin antimikrobiyal aktiviteleri, Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Staphylococcus aureus suşları ve Candida albicans mayası üzerinde test edildi. Ecs, MIC olarak 16.2 ve 129.3 mg mL-1 arasında antimikrobiyal aktivite sergilerken, EcAgNP, 6.31 ve 14.65 μg mL-1 arasında MIC gösterdi. A549, HT29 ve MDA-MB-231 kanser hücre hatlarında Ecs ve EcAgNP'lerin sitotoksisitesi, MTT ile test edildi. EcAgNPs MDA-MB-231, HT29 ve A549 kanser hücre hatları üzerinde (8.10±0.01, 5.08±0.01 and 18.58±0.03 μg mL-1) Ecs'ye (219.70±0.73, 916.24±0.67, 999.30±1.86 μg mL-1) göre daha dikkate değer antikanser aktivite sergilemiştir ve çeşitli terapötiklerde kullanımı için araştırılmalıdır.

GREEN SYNTHESIS, ANTIMICROBIAL AND ANTICANCER ACTIVITIES OF AgNPs PREPARED FROM THE LEAF EXTRACT OF Eucalyptus camaldulensis

Herein we report the green synthesis of silver nanoparticles (EcAgNPs) from water fraction (Ecs), obtained from methanol extract of Eucalyptus camaldulensis at room conditions. UV-vis, HR-TEM and SEM-EDS devices were used to characterize the EcAgNPs. The UV-visible spectrum of EcAgNPs showed surface plasmon resonance peak at 420 nm. HR-TEM images showed that the EcAgNPs were spherical with a diameter in the range 3.7-29.6 nm. Ag+ ions were reduced to Ago by phytochemicals in the without adding external reducing agent. The presence of biomolecules in the Ecs was investigated by UPLC-MS/MS that detected gallic acid (20.26±0.10 ppb) and quercetin (12.4±0.08 ppb) as major constituents. The antimicrobial activities of Ecs and synthesized EcAgNPs were tested on Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Staphylococcus aureus strains and Candida albicans yeast. Ecs exhibited antimicrobial activity as MIC between 16.2 and 129.3 mg mL-1 while EcAgNP showed MIC between 6.31 and 14.65 μgmL-1. The cytotoxicity of both Ecs and EcAgNPs on A549, HT29 and MDA-MB-231 cancer cell lines was tested by MTT. EcAgNPs showed more significant anticancer activity on MDA-MB-231, HT29 and A549 cancer cell lines (8.10±0.01, 5.08±0.01 and 18.58±0.03 μg mL-1) than Ecs (219.70±0.73, 916.24±0.67, 999.30±1.86 μg mL-1) and it should be investigated for use in various therapeutics.

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