Cevahir ALTINKAYNAK, İsmail ÖÇSOY, Nalan ÖZDEMİR

Bu çalışmada, mükemmel şekilde güçlenmiş aktivite, stabilite, tekrarkullanılabilirlik ve sulu çözeltilerden fenol gideriminde kullanılacak yaban turpu peroksidaz (HRP), bakır iyonları (Cu2+) ve demir oksit nanoparçacıklarından (Fe3O4 NP'ler) oluşan manyetik hibrit nano çiçeklerin (MhNF) hazırlanması rapor edildi. Önceden sentezlenmiş küçük boyutlu Fe3O4 NP'lar, sentezlenen HRP-Cu2+ hibrit nano çiçeklere (hNF'ler) immobilize edilmiştir. Sentezlenen MhNF'ler SEM, EDX ve XRD analizi kullanılarak karakterize edildi. Ayrıca FTIR kullanılarak serbest HRP ve MhNF'nin kimyasal yapıları da karakterize edildi. Sentezlenen MhNF'lerin protein kapsülleme verimleri spektrofotometrik olarak belirlendi ve tüm sentez koşulları için ~%98 olarak hesaplandı. Serbest HRP, HRP bazlı manyetik olmayan hibrit nano çiçek (hNF) ve MhNF'nin aktiviteleri sırasıyla 5847.6, 16738.2 ve 18830.5 EU/mg olarak hesaplanmıştır. Sentezlenen MhNF'lerin fenol giderimi için optimum pH'ı 7 olarak belirlendi. MhNF, tekrar kullanılmak üzere harici bir manyetik alan oluşturularak reaksiyon ortamından kolaylıkla ve hızla ayrıldı. Yedi döngüden sonra bile, MhNF'ler başlangıç aktivitelerinin yaklaşık %75'inden fazlasını korudu. Tekrarlanan kullanımın sermaye ve işletme maliyetlerini azaltabileceğine inanıyoruz. Sonuçlar, özellikle atık su arıtımı için endüstriyel uygulamada HRP'nin verimliliğinin ve uygulanabilirliğinin arttırılması açısından çok umut vericidir.

Anahtar Kelimeler:

enzim aktivite, manyetik hibrit nanoçiçek, peroksidaz, fenol giderimi, Enzim-inorganik hibrit nanoçiçek

A RATIONAL SYNTHESIS OF MAGNETIC NANOPARTICLES INCORPORATED HORSERADISH PEROXIDASE NANOFLOWER AND ITS USE FOR THE REMOVAL OF PHENOL THROUGH OXIDATIVE COUPLING REACTION WITH GREAT REUSABILITY

Herein, we report preparation of magnetic hybrid nanoflower (MhNF) formed of horseradish peroxidase (HRP), copper ions (Cu2+) and iron oxide nanoparticles (Fe3O4 NPs) with greatly enhanced activity, stability, reusability and applicability for use in removal of phenol from aqueous solution. In this study, pre-synthesized small sized Fe3O4 NPs were immobilized to synthesized HRP-Cu2+ hybrid nanoflower (hNFs). The synthesized MhNFs were characterized using SEM, EDX and XRD analysis. We also characterized the chemical structures of free HRP and MhNF using FTIR. The protein encapsulation yields of synthesized MhNFs were determined spectrophotometrically and calculated as ~98% for all synthesis conditions. The activities of free HRP, HRP-based non-magnetic hybrid nanoflower (hNF) and MhNF were calculated as 5847.6, 16738.2, and 18830.5 EU/mg, respectively. The optimum pH of the synthesized MhNFs for phenol removal was determined as pH 7. MhNF was easily and rapidly separated from reaction medium by an external magnetic field to be re-used. Even after seven cycles, MhNFs maintained more than 75% of their initial activity. We believe that the repeated use can reduce the capital and operation costs. The results are very promising in terms of increasing efficiency and applicability of HRP in industrial application especially for wastewater treatment.

Keywords:

Enzyme-inorganic hybrid nanoflower, enzyme activity, magnetic hybrid nanoflower, peroxidase, phenol removal,

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