Response surface methods (RSM) have some advantages such as easy optimization, to reach results quickly, and availability of evaluation statistically. Because of these reasons, the experiments were designed by using RSM. In this study, copper extraction was investigated from advanced milled chalcopyrite concentrate by using hydrogen peroxide as oxidant and acetic acid as stabilizer. In this context, effects of different parameters were studied such as grinding time, leaching time, peroxide concentration, and leaching temperature. Results showed that nearly 100% of copper was extracted compatible statistically (R2=0.8072) while the most effective parameter was defined the changing of hydrogen peroxide concentration